Publication: Genetic polymorphisms of major house dust mite allergens
1
Issued Date
2006-04-01
Resource Type
ISSN
13652222
09547894
09547894
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2-s2.0-33645323782
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Mahidol University
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SCOPUS
Bibliographic Citation
Clinical and Experimental Allergy. Vol.36, No.4 (2006), 510-516
Suggested Citation
S. Piboonpocanun, N. Malainual, O. Jirapongsananuruk, P. Vichyanond, W. R. Thomas Genetic polymorphisms of major house dust mite allergens. Clinical and Experimental Allergy. Vol.36, No.4 (2006), 510-516. doi:10.1111/j.1365-2222.2006.02464.x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/23344
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Title
Genetic polymorphisms of major house dust mite allergens
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Abstract
Background: Polymorphic sequence substitutions in the major mite allergens can markedly affect immunoglobulin E binding and T cell responses, but there are few studies on environmental isolates from Dermatophagoides pteronyssinus and none for D. farinae. Objective: To determine the sequence variation of the group 1 and 2 allergens from environmental D. pteronyssinus and D. farinae. Methods: RNA from each species was isolated from homes in Bangkok and the sequence of Der p 1, Der p 2, Der f 1, and Der f 2 determined from cDNA produced by high fidelity polymerase chain reactions. Results: The enlarged data set revealed preferred amino acid substitutions in residues 19, 81, and 215 of Der p 1 as well as sporadic changes. Der p 2 showed frequent variations with clusters of amino acid substitutions, but the canonical Der p 2.0101 was not found in any of 17 sequences. Der f 2 showed variants with clusters of substitutions similar to Der p 2 but in different amino acid positions and without any structural concordance. Der f 1 in contrast to the other allergens had few amino acid sequence substitutions. Conclusions: The sequence information on variants provides data important for the optimal design of allergen formulations and useful for the genetic engineering and structure-function analyses of the major allergens. © 2006 Blackwell Publishing Ltd.