Publication: Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child Transmission
Issued Date
2006-09-01
Resource Type
ISSN
01434004
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2-s2.0-33747155554
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Mahidol University
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SCOPUS
Bibliographic Citation
Placenta. Vol.27, No.9-10 (2006), 989-995
Suggested Citation
S. Pornprasert, A. Faye, J. Y. Mary, G. Dolcini, P. Leechanachai, G. Chaouat, N. Ngo, F. Barré-Sinoussi, E. Menu Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child Transmission. Placenta. Vol.27, No.9-10 (2006), 989-995. doi:10.1016/j.placenta.2005.10.010 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/23637
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Title
Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child Transmission
Abstract
Mechanisms of HIV-1 in utero mother-to-child transmission (MTCT) protection provided by AZT are not completely understood. The placental cytokine network is involved in the control of HIV-1 in utero transmission but the effect of AZT on this network is unknown. To evaluate the effects of AZT on placental cytokine expression, the chorionic villi from HIV-1 uninfected women term placentae were cultured with 0, 100, and 2000 ng/ml AZT. Tissue fragments were harvested at days 1, 4, and 7 to determine the level of cytokine mRNA by real-time RT-PCR. The viability and morphology of the placental histocultures were monitored by the expression of beta-human chorionic gonadotropin (β-hCG) gene, lipopolysaccharide (LPS) activation, and microscopic examination. AZT at 2000 ng/ml significantly down-regulated TNF-α mRNA expression at day 1 and day 4, but had no effect on β-hCG, stromal cell-derived factor 1 (SDF-1), and IL-10 gene expression. AZT did not induce any deleterious impact on placental tissue structure. Furthermore, activation of chorionic villi by LPS for 24 h up-regulated IL-10 and TNF-α mRNA expression. Down-regulation of TNF-α mRNA could represent a mechanism through which AZT can decrease the risk of HIV-1 MTCT, in addition to its direct effect on HIV-1 replication. © 2005 Elsevier Ltd. All rights reserved.