Publication: EDTA-induced membrane fluidization and destabilization: Biophysical studies on artificial lipid membranes
Issued Date
2007-11-01
Resource Type
ISSN
17457270
16729145
16729145
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2-s2.0-36249021386
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Mahidol University
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SCOPUS
Bibliographic Citation
Acta Biochimica et Biophysica Sinica. Vol.39, No.11 (2007), 901-913
Suggested Citation
Virapong Prachayasittikul, Chartchalerm Isarankura-Na-Ayudhya, Tanawut Tantimongcolwat, Chanin Nantasenamat, Hans Joachim Galla EDTA-induced membrane fluidization and destabilization: Biophysical studies on artificial lipid membranes. Acta Biochimica et Biophysica Sinica. Vol.39, No.11 (2007), 901-913. doi:10.1111/j.1745-7270.2007.00350.x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/24088
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Title
EDTA-induced membrane fluidization and destabilization: Biophysical studies on artificial lipid membranes
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Abstract
The molecular mechanism of ethylenediaminetetraacetic acid (EDTA)-induced membrane destabilization has been studied using a combination of four biophysical techniques on artificial lipid membranes. Data from Langmuir film balance and epifluorescence microscopy revealed the fluidization and expansion effect of EDTA on phase behavior of monolayers of either 1,2-dipalmitoyl-sn- glycero-3-phosphocholine (DPPC) or mixtures of DPPC and metal-chelating lipids, such as Nα, Nα-Bis[carboxymethyl]-N ε-[(dioctadecylamino)succinyl]-L-lysine or 1,2-dioleoyl-sn- glycero-3-[N-(5-amino-1-carboxypentyl iminodiacetic acid) succinyl]. A plausible explanation could be drawn from the electrostatic interaction between negatively charged groups of EDTA and the positively charged choline head group of DPPC. Intercalation of EDTA into the lipid membrane induced membrane curvature as elucidated by atomic force microscopy. Growth in size and shape of the membrane protrusion was found to be time-dependent upon exposure to EDTA. Further loss of material from the lipid membrane surface was monitored in real time using a quartz crystal microbalance. This indicates membrane restabilization by exclusion of the protrusions from the surface. Loss of lipid components facilitates membrane instability, leading to membrane permeabilization and lysis. © 2007 Institute of Biochemistry and Cell Biology, SIBS, CAS.