Publication: Utility of selected non-coding chloroplast DNA sequences for lineage assessment of Musa interspecific hybrids
Issued Date
2007-07-01
Resource Type
ISSN
02191024
12258687
12258687
Other identifier(s)
2-s2.0-34548022143
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Biochemistry and Molecular Biology. Vol.40, No.4 (2007), 577-587
Suggested Citation
Sasivimon Swangpol, Hugo Volkaert, Rachel C. Sotto, Tosak Seelanan Utility of selected non-coding chloroplast DNA sequences for lineage assessment of Musa interspecific hybrids. Journal of Biochemistry and Molecular Biology. Vol.40, No.4 (2007), 577-587. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/24174
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Utility of selected non-coding chloroplast DNA sequences for lineage assessment of Musa interspecific hybrids
Abstract
Single-copy chloroplast loci are used widely to infer phylogenetic relationship at different taxonomic levels among various groups of plants. To test the utility of chloroplast loci and to provide additional data applicable to hybrid evolution in Musa, we sequenced two introns, rpl16 and ndhA, and two intergenic spacers, psaA-ycf3 and petA-psbJ-psbL-psbF and combined these data. Using these four regions, Musa acuminata Colla (A)- and M. balbisiana Colla (B)-containing genomes were clearly distinguished. Some triploid interspecific hybrids contain A-type chloroplasts (the AAB/ABB) while others contain B-type chloroplasts (the BBA/BBB). The chloroplasts of all cultivars in 'Namwa' (BBA) group came from the same wild maternal origin, but the specific parents are still unrevealed. Though, average sequence divergences in each region were little (less than 2%), we propose that petA-psbJ intergenic spacer could be developed for diversity assessment within each genome. This segment contains three single nucleotide polymorphisms (SNPs) and two indels which could distinguish diversity within A genome whereas this same region also contains one SNP and an indel which could categorize B genome. However, an inverted repeat region which could form hairpin structure was detected in this spacer and thus was omitted from the analyses due to their incongruence to other regions. Until thoroughly identified in other members of Musaceae and Zingiberales clade, utility of this inverted repeat as phylogenetic marker in these taxa are cautioned.