Publication: Protection of cells from oxidative stress by microsomal glutathione transferase 1
1
Issued Date
2007-04-06
Resource Type
ISSN
10902104
0006291X
0006291X
Other identifier(s)
2-s2.0-33847169447
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.355, No.2 (2007), 592-596
Suggested Citation
Atchasai Siritantikorn, Katarina Johansson, Karin Åhlen, Rosanna Rinaldi, Tuangporn Suthiphongchai, Prapon Wilairat, Ralf Morgenstern Protection of cells from oxidative stress by microsomal glutathione transferase 1. Biochemical and Biophysical Research Communications. Vol.355, No.2 (2007), 592-596. doi:10.1016/j.bbrc.2007.02.018 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/24211
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Title
Protection of cells from oxidative stress by microsomal glutathione transferase 1
Abstract
Rat liver microsomal glutathione transferase 1 (MGST1) is a membrane-bound enzyme that displays both glutathione transferase and glutathione peroxidase activities. We hypothesized that physiologically relevant levels of MGST1 is able to protect cells from oxidative damage by lowering intracellular hydroperoxide levels. Such a role of MGST1 was studied in human MCF7 cell line transfected with rat liver mgst1 (sense cell) and with antisense mgst1 (antisense cell). Cytotoxicities of two hydroperoxides (cumene hydroperoxide (CuOOH) and hydrogen peroxide) were determined in both cell types using short-term and long-term cytotoxicity assays. MGST1 significantly protected against CuOOH and against hydrogen peroxide (although less pronounced and only in short-term tests). These results demonstrate that MGST1 can protect cells from both lipophilic and hydrophilic hydroperoxides, of which only the former is a substrate. After CuOOH exposure MGST1 significantly lowered intracellular ROS as determined by FACS analysis. © 2007 Elsevier Inc. All rights reserved.