Publication: Prognostic value of DNA alterations on chromosome 17p13.2 for intrahepatic cholangiocarcinoma
Issued Date
2007-06-07
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ISSN
10079327
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2-s2.0-34447093833
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Mahidol University
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SCOPUS
Bibliographic Citation
World Journal of Gastroenterology. Vol.13, No.21 (2007), 2986-2991
Suggested Citation
Ubol Chuensumran, Sopit Wongkham, Chawalit Pairojkul, Siri Chauin, Songsak Petmitr Prognostic value of DNA alterations on chromosome 17p13.2 for intrahepatic cholangiocarcinoma. World Journal of Gastroenterology. Vol.13, No.21 (2007), 2986-2991. doi:10.3748/wjg.v13.i21.2986 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/24845
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Title
Prognostic value of DNA alterations on chromosome 17p13.2 for intrahepatic cholangiocarcinoma
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Abstract
Aim: To characterize and evaluate DNA alterations among intrahepatic cholangiocarcinoma (ICC) patients. Methods: DNA from tumor and corresponding normal tissues of 52 patients was amplified with 33 arbitrary primers. The DNA fragment that alters most frequently in ICC was cloned, sequenced, and identified by comparison with known nucleotide sequences in the genome database (www.ncbi.nlm.nih.gov). The DNA copy numbers of the allelic alterations in cholangiocarcinoma were determined by quantitative real-time PCR and interpreted as allelic loss or DNA amplification by comparison with the reference gene. Associations between allelic imbalance and clinicopathological parameters of ICC patients were evaluated by χ2-test. The Kaplan-Meier method was used to analyze survival rates. Results: From 33 primers, an altered DNA fragment (518 bp) amplified from BC17 random primer was found frequently in the tumors analyzed and mapped to chromosome 17p13.2. Sixteen of 52 (31%) cases showed DNA amplification, while 7 (13%) showed allelic loss. Interestingly, DNA amplification on chromosome 17p13.2 was associated with a good prognosis, median survival time (wk) of amp vs no amp was 44.14 vs 24.14, P = 0.002; whereas allelic loss of this DNA sequence corresponded with a poor prognosis, median survival time (wk) of loss vs no loss was 18.00 vs 28.71, P = 0.019). Moreover, Kaplan-Meier curves comparing the DNA alterations with survival depicted highly significant separation that the median survival time equal to DNA amplification, allelic loss, and normal was 44.14 wk, 18.00 wk, and 24.29 wk, respectively (P = 0.005). Conclusion: Alterations in the DNA sequence on chromosome 17p13.2 may be involved in cholangiocarcinogenesis, and could be used as a prognostic marker in the treatment of ICC patients. © 2007 The WJG Press. All rights reserved.