Publication: Molecular analysis of Mycobacterium tuberculosis causing multidrug-resistant tuberculosis meningitis
Issued Date
2007-02-01
Resource Type
ISSN
10273719
Other identifier(s)
2-s2.0-33846861289
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Tuberculosis and Lung Disease. Vol.11, No.2 (2007), 202-208
Suggested Citation
Maxine Caws, G. E. Thwaites, P. M. Duy, D. Q. Tho, N. T. Ngoc Lan, D. V. Hoa, T. T. Hong Chau, M. N. Thu Huyen, P. T. Hoang Anh, N. V.V. Chau, N. T. Chinh, K. Stepniewska, J. Farrar Molecular analysis of Mycobacterium tuberculosis causing multidrug-resistant tuberculosis meningitis. International Journal of Tuberculosis and Lung Disease. Vol.11, No.2 (2007), 202-208. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/25015
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Title
Molecular analysis of Mycobacterium tuberculosis causing multidrug-resistant tuberculosis meningitis
Abstract
SETTING: Tertiary referral hospitals in southern Vietnam. OBJECTIVE: Molecular characterisation of multidrug-resistant (MDR) tuberculous meningitis (TBM). DESIGN: Mycobacterium tuberculosis isolates from the cerebrospinal fluid (CSF) of 198 Vietnamese adults were compared with 237 isolates from patients with pulmonary tuberculosis (PTB) matched for age, sex and residential district. Isolates resistant to isoniazid or rifampicin (RMP) were sequenced in the rpoB and katG genes, inhA promoter and oxyR-ahpC intergenic regions. RESULTS: While drug resistance rates were lower in the CSF (2.5% MDR) than pulmonary isolates (5.9% MDR), the difference was not significant. The most commonly mutated codons were 531, 526 and 516 in rpoB and 315 in katG. Four novel triple mutants in rpoB were identified. CONCLUSION: RMP resistance is a good surrogate marker for MDR-TBM in this setting. However, probes directed against these three codons would have a maximum sensitivity of only 65%. A rapid phenotypic detection test may be more applicable for the diagnosis of MDR-TBM. © 2007 The Union.