Publication: Stable marker on flagellin gene sequences related to arabinose non- assimilating pathogenic Burkholderia pseudomallei
Issued Date
1999-01-01
Resource Type
ISSN
03855600
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2-s2.0-0032724426
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Mahidol University
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SCOPUS
Bibliographic Citation
Microbiology and Immunology. Vol.43, No.11 (1999), 995-1001
Suggested Citation
Sumet Wajanarogana, Piengchan Sonthayanon, Vanaporn Wuthiekanun, Sakol Panyim, Andrew J.H. Simpson, Sumalee Tungpradabkul Stable marker on flagellin gene sequences related to arabinose non- assimilating pathogenic Burkholderia pseudomallei. Microbiology and Immunology. Vol.43, No.11 (1999), 995-1001. doi:10.1111/j.1348-0421.1999.tb01238.x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/25449
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Title
Stable marker on flagellin gene sequences related to arabinose non- assimilating pathogenic Burkholderia pseudomallei
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Abstract
Using PCR-based isolation and sequence analysis of the flagellin gene from two distinct biotypes of Burkholderia pseudomallei, a 15-bp deletion was found within the variable domain of the gene in isolates capable of assimilating arabinose (Ara+). This finding led to the development of a PCR- based method in order to differentiate and identify pathogenic B. pseudomallei for epidemiological study. A pair of specific primers was designed covering the 15-bp deletion region at the variable domain. PCR- amplification products of 176 and 191 bp in size were detected from 41 Ara+ isolates and 39 Ara- isolates of B. pseudomallei, respectively. Moreover, flagellin gene fragments of other bacterial species tested in this study were not amplified using these primers. The results suggest that the flagellin gene sequences of both B. pseudomallei biotypes in this region are stable and distinct. This method can be applied and useful for the epidemiological study of B. pseudomallei.