Publication:
Identification of circulating antibodies in fasciolosis and localization of 66 kDa antigenic target using monoclonal antibodies

1

Issued Date

1999-03-01

Resource Type

Article

ISSN

0125877X

Other identifier(s)

2-s2.0-0033030457

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Asian Pacific Journal of Allergy and Immunology. Vol.17, No.1 (1999), 53-58

Suggested Citation

D. Krailas, V. Viyanant, P. Ardseungnoen, P. Sobhon, E. S. Upatham, R. Keawjam Identification of circulating antibodies in fasciolosis and localization of 66 kDa antigenic target using monoclonal antibodies. Asian Pacific Journal of Allergy and Immunology. Vol.17, No.1 (1999), 53-58. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/25670

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Title

Identification of circulating antibodies in fasciolosis and localization of 66 kDa antigenic target using monoclonal antibodies

Author(s)

D. Krailas
 V. Viyanant
 P. Ardseungnoen
 P. Sobhon
 E. S. Upatham
 R. Keawjam

Other Contributor(s)

Mahidol University

Abstract

We identified three specific circulating antibodies in serum of cattle naturally infected with Fasciola gigantica. Two of the antibodies were found to react specifically to 97 and 66 kDa antigenic molecules of adult worm tegumental membrane extract. The third antibody was identified by the reaction with 26-28 kDa molecule of the excretory/secretory antigens. Monoclonal antibody against 66 kDa protein was developed and used for localization of its antigenic target in adult worm frozen sections. The experiment demonstrated that 66 kDa protein is a component on the outer surface membrane and on the membrane lining of the caecal epithelial of adult worm. The 66 kDa antigen was considered as a promising candidate for immunodiagnosis and vaccine.

Keyword(s)

Medicine

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https://repository.li.mahidol.ac.th/handle/123456789/25670

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Scopus 1991-2000