Publication: Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone
Issued Date
2009-05-01
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ISSN
09486143
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2-s2.0-64049094651
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Mahidol University
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SCOPUS
Bibliographic Citation
Histochemistry and Cell Biology. Vol.131, No.5 (2009), 629-642
Suggested Citation
Sasiporn Panasophonkul, Somjai Apisawetakan, Scott F. Cummins, Patrick S. York, Bernard M. Degnan, Peter J. Hanna, Porncharn Saitongdee, Prasert Sobhon, Prapee Sretarugsa Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone. Histochemistry and Cell Biology. Vol.131, No.5 (2009), 629-642. doi:10.1007/s00418-009-0555-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/27236
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Title
Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone
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Abstract
In molluscs, the neurotransmitter serotonin (5-HT) has been linked to a variety of biological roles including gamete maturation and spawning. The possible involvement of 5-HT in abalone gamete release was demonstrated by a dose-dependent increase in Haliotis rubra gonad contractile bioactivity following 5-HT stimulation. Physiological functions associated with 5-HT, are mediated through binding to 5-HT receptors. A cDNA encoding a putative 5-HT receptor consisting of 359 amino acids was isolated from the tropical abalone H. asinina, termed 5-HT1 ha. The 5-HT1 hashares G-protein-coupled receptor motifs with metazoan 5-HT receptors, including predicted transmembrane domains, active sites for protein kinase action, and N-linked glycosylation sites. However, the third intracellular loop of 5-HT1 hais relatively short, and only six transmembrane domains are predicted, implying a truncated receptor. Phylogenetic analysis with known 5-HT receptor genes suggests that 5-HT1 habelongs to the type 1 5-HT receptor family. Expression analysis by RT-PCR showed that 5-HT1 hamRNA was present in all tissues examined, including the neural ganglia and gonad tissues. Immunocytochemistry revealed the presence of 5-HT1 haspecifically within the soma of neuronal cells located in the outer cortex of both cerebral and pleuropedal ganglia. In ovarian and testicular tissues, 5-HT1 haimmunoreactivity was observed in epithelial cells of the outer capsule and connective tissue of the trabeculae to which the gamete follicles adhere. Whether this receptor transcript is translated to a functional protein needs to be verified, but if so, it could play a role in reproduction. © 2009 Springer-Verlag.