Publication: CD2068 potentially mediates multidrug efflux in Clostridium difficile
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Issued Date
2017
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eng
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Mahidol University
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Scopus
Bibliographic Citation
Scientific Reports. Vol.7, No.1 (2017), 9982
Suggested Citation
Chawalit Ngernsombat, Suthasinee Sreesai, Phurt Harnvoravongchai, Surang Chankhamhaengdecha, Tavan Janvilisri CD2068 potentially mediates multidrug efflux in Clostridium difficile. Scientific Reports. Vol.7, No.1 (2017), 9982. doi:10.1038/s41598-017-10155-x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/2835
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Title
CD2068 potentially mediates multidrug efflux in Clostridium difficile
Abstract
Clostridium difficile is a major cause of antibiotic-associated diarrhea and the treatment thereof
becomes more difficult owing to a rise of multidrug resistant strains. ATP-binding cassette (ABC)
transporters are known to play a crucial role in the resistance to multiple antibiotics. In this study,
the potential contribution of an ABC transporter in C. difficile multidrug resistance was investigated.
The expression level of the cd2068 gene in C. difficile encoding an ABC transporter was up-regulated
following the exposure to certain antibiotics compared to the control cells. Heterologous expression of
CD2068 in Escherichia coli revealed that it mediated the efflux of fluorescent substrates and conferred
resistance to multiple drugs. The CD2068-associated ATPase activity in membrane vesicles was
also stimulated by various antibiotics. Furthermore, the insertional inactivation of the cd2068 gene
in C. difficile led to a significant increase in susceptibility to antibiotics, which could be genetically
complemented, supporting that CD2068 was directly associated to the drug resistance. These results
demonstrate the potential role for the ABC transporter CD2068 in the resistance mechanism against
multiple drugs in C. difficile.