Publication: A Burkholderia pseudomallei protein microarray reveals serodiagnostic and cross-reactive antigens
Issued Date
2009-08-11
Resource Type
ISSN
10916490
00278424
00278424
Other identifier(s)
2-s2.0-69449101951
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Mahidol University
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SCOPUS
Bibliographic Citation
Proceedings of the National Academy of Sciences of the United States of America. Vol.106, No.32 (2009), 13499-13504
Suggested Citation
Philip L. Felgner, Matthew A. Kayala, Adam Vigil, Chad Burk, Rie Nakajima-Sasaki, Jozelyn Pablo, Douglas M. Molina, Siddiqua Hirst, Janet S.W. Chew, Dongling Wang, Gladys Tan, Melanie Duffield, Ron Yang, Julien Neel, Narisara Chantratita, Greg Bancroft, Ganjana Lertmemongkolchai, D. Huw Davies, Pierre Baldi, Sharon Peacock, Richard W. Titball A Burkholderia pseudomallei protein microarray reveals serodiagnostic and cross-reactive antigens. Proceedings of the National Academy of Sciences of the United States of America. Vol.106, No.32 (2009), 13499-13504. doi:10.1073/pnas.0812080106 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/28387
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Title
A Burkholderia pseudomallei protein microarray reveals serodiagnostic and cross-reactive antigens
Author(s)
Philip L. Felgner
Matthew A. Kayala
Adam Vigil
Chad Burk
Rie Nakajima-Sasaki
Jozelyn Pablo
Douglas M. Molina
Siddiqua Hirst
Janet S.W. Chew
Dongling Wang
Gladys Tan
Melanie Duffield
Ron Yang
Julien Neel
Narisara Chantratita
Greg Bancroft
Ganjana Lertmemongkolchai
D. Huw Davies
Pierre Baldi
Sharon Peacock
Richard W. Titball
Matthew A. Kayala
Adam Vigil
Chad Burk
Rie Nakajima-Sasaki
Jozelyn Pablo
Douglas M. Molina
Siddiqua Hirst
Janet S.W. Chew
Dongling Wang
Gladys Tan
Melanie Duffield
Ron Yang
Julien Neel
Narisara Chantratita
Greg Bancroft
Ganjana Lertmemongkolchai
D. Huw Davies
Pierre Baldi
Sharon Peacock
Richard W. Titball
Abstract
Understanding the way in which the immune system responds to infection is central to the development of vaccines and many diagnostics. To provide insight into this area, we fabricated a protein microarray containing 1,205 Burkholderia pseudomallei proteins, probed it with 88 melioidosis patient sera, and identified 170 reactive antigens. This subset of antigens was printed on a smaller array and probed with a collection of 747 individual sera derived from 10 patient groups including melioidosis patients from Northeast Thailand and Singapore, patients with different infections, healthy individuals from the USA, and from endemic and nonendemic regions of Thailand. We identified 49 antigens that are significantly more reactive in melioidosis patients than healthy people and patients with other types of bacterial infections. We also identified 59 cross-reactive antigens that are equally reactive among all groups, including healthy controls from the USA. Using these results we were able to devise a test that can classify melioidosis positive and negative individuals with sensitivity and specificity of 95% and 83%, respectively, a significant improvement over currently available diagnostic assays. Half of the reactive antigens contained a predicted signal peptide sequence and were classified as outer membrane, surface structures or secreted molecules, and an additional 20% were associated with pathogenicity, adaptation or chaperones. These results show that microarrays allow a more comprehensive analysis of the immune response on an antigen-specific, patient-specific, and population-specific basis, can identify serodiagnostic antigens, and contribute to a more detailed understanding of immunogenicity to this pathogen.