Publication: Comparison between Pseudomonas aeruginosa siderophores and desferrioxamine for iron acquisition from ferritin
Issued Date
2010-08-01
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19057415
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2-s2.0-77957961895
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Biomedicine. Vol.4, No.4 (2010), 631-635
Suggested Citation
Somporn Srifuengfung, Susan Assanasen, Malulee Tuntawiroon, Sumonrat Meejanpetch Comparison between Pseudomonas aeruginosa siderophores and desferrioxamine for iron acquisition from ferritin. Asian Biomedicine. Vol.4, No.4 (2010), 631-635. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/28658
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Title
Comparison between Pseudomonas aeruginosa siderophores and desferrioxamine for iron acquisition from ferritin
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Abstract
Background: Siderophore is an iron chelator produced by microorganism. Pseudomonas aeruginosa produces two siderophores (pyoverdin and pyochelin). Desferrioxamine is a siderophore used in thalassemia patients to treat an iron overload of vital organs. Objective: Compare the ability of pyoverdin, pyochelin, and desferrioxamine for iron mobilization from ferritin. Materials and Methods: In vitro experiment, the ability of P. aeruginosa siderophores and desferrioxamine for iron mobilization from ferritin was compared by using a dialysis membrane assay at pH values of 7.4 and 6.0. Stimulation of P. aeruginosa PAO1 growth by all siderophores was studied in glucose minimum medium. Results: All three compounds were capable of iron mobilization at both pHs. At pH 6.0, the most effectiveness compound was desferrioxamine (31.6%), followed by pyoverdin (21.5%) and pyochelin (13.7%) compared on weight basis, each at 10 μg/mL. At equimolar concentration, their activities were desferrioxamine (38.5 ±1.2%), followed by pyoverdin (32.0 ±4.8%) and pyochelin (26.7 ±1.9%), respectively. Conclusion: The most effective compound in iron mobilization from ferritin was desferrioxamine, followed by pyoverdin and pyochelin respectively.