Publication: NGAL knockdown by siRNA in human cholangiocarcinoma cells suppressed invasion by reducing NGAL/MMP-9 complex formation
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Issued Date
2010-05-01
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ISSN
15737276
02620898
02620898
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2-s2.0-77953957362
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Mahidol University
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SCOPUS
Bibliographic Citation
Clinical and Experimental Metastasis. Vol.27, No.5 (2010), 295-305
Suggested Citation
Chalinee Nuntagowat, Kawin Leelawat, Rutaiwan Tohtong NGAL knockdown by siRNA in human cholangiocarcinoma cells suppressed invasion by reducing NGAL/MMP-9 complex formation. Clinical and Experimental Metastasis. Vol.27, No.5 (2010), 295-305. doi:10.1007/s10585-010-9327-y Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/28721
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Title
NGAL knockdown by siRNA in human cholangiocarcinoma cells suppressed invasion by reducing NGAL/MMP-9 complex formation
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Abstract
We studied the role of Neutrophil Gelatinase-associated Lipocalin (NGAL, lipocalin 2) in regulating the invasiveness of a cholangiocarcinoma cell line, RMCCA-1. RMCCA-1 cells expressed multiple forms of 25, 40, 75 and 115/135 kDa NGAL which were detected in the conditioned medium, whereas only the 25 kDa form was detected in the cell lysates. NGAL expression was induced by serum deprivation. NGAL downregulation by siRNA suppressed NGAL mRNA and protein expression by about 70-80%, concommittant with a significant reduction of in vitro invasiveness, migration and pro-MMP-9 activity, but not cell proliferation. Suppression of pro-MMP-9 activity paralleled a reduction of NGAL/MMP-9 complex in the conditioned medium, although MMP-9 mRNA expression was unaffected. Our data suggest that NGAL promotes the invasiveness of the cholangiocarcinoma cells by forming complex with MMP-9, stabilizing its activity and rendering the cancer cells to be more invasive. © 2010 Springer Science+Business Media B.V.