Publication: Characterization of binding and bioaccessibility of Cr in Cr-enriched yeast by sequential extraction followed by two-dimensional liquid chromatography with mass spectrometric detection
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Issued Date
2010-01-01
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ISSN
16182650
16182642
16182642
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2-s2.0-76749148964
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Mahidol University
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SCOPUS
Bibliographic Citation
Analytical and Bioanalytical Chemistry. Vol.396, No.3 (2010), 1355-1364
Suggested Citation
Nattikarn Kaewkhomdee, Sandra Mounicou, Joanna Szpunar, Ryszard Lobinski, Juwadee Shiowatana Characterization of binding and bioaccessibility of Cr in Cr-enriched yeast by sequential extraction followed by two-dimensional liquid chromatography with mass spectrometric detection. Analytical and Bioanalytical Chemistry. Vol.396, No.3 (2010), 1355-1364. doi:10.1007/s00216-009-3285-8 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/28833
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Title
Characterization of binding and bioaccessibility of Cr in Cr-enriched yeast by sequential extraction followed by two-dimensional liquid chromatography with mass spectrometric detection
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Abstract
Sequential extraction (water, Driselase, protease XIV) and extraction with simulated gastric and intestinal fluids were proposed to characterize the binding and the bioaccessibility of chromium in two commercial food supplements obtained by incorporation of this element into yeast. Chromium in Cr-enriched yeast was found to be hardly extractable with water, Driselase, or simulated gastric fluid (recoveries of approximately 10-20%), but proteolysis or gastrointestinal fluid digestion released more than half of the chromium present. Fractionation with size-exclusion chromatography with Cr-specific detection by inductively coupled plasma mass spectrometry (ICP MS) allowed the distinction of two fractions: one below approximately 1 kDa and one 1-5 kDa; they contained the entirety of the released Cr with proportions varying as a function of the extracting solution and the origin of sample. When collected and investigated by reversed-phase high-performance liquid chromatography-ICP MS, the low molecular mass fraction was found to release Cr(III), whereas the heavier one showed most of Cr bound in fairly stable hydrophobic complexes. However, an attempt of their identification by electrospray ionization MS/MS and matrix-assisted laser desorption ionization MS was not successful. © 2009 Springer-Verlag.