Publication: Measuring binding affinity of protein-ligand interaction using spectrophotometry: Binding of neutral red to riboflavin-binding protein
2
Issued Date
2010-08-27
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ISSN
00219584
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2-s2.0-77955912880
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Chemical Education. Vol.87, No.8 (2010), 829-831
Suggested Citation
Pirom Chenprakhon, Jeerus Sucharitakul, Bhinyo Panijpan, Pimchai Chaiyen Measuring binding affinity of protein-ligand interaction using spectrophotometry: Binding of neutral red to riboflavin-binding protein. Journal of Chemical Education. Vol.87, No.8 (2010), 829-831. doi:10.1021/ed1000125 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/28928
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Title
Measuring binding affinity of protein-ligand interaction using spectrophotometry: Binding of neutral red to riboflavin-binding protein
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Abstract
The dissociation constant, Kd, of the binding of riboflavin-binding protein (RP) with neutral red (NR) can be determined by titrating RP to a fixed concentration of NR. Upon adding RP to the NR solution, the maximum absorption peak of NR shifts to 545 nm from 450 nm for the free NR. The change of the absorption can be used to determine the Kdby plotting an absorbance ratio versus the concentration of free RP. The data are analyzed via a nonlinear fitting analysis to yield a Kdof 2.2 ± 0.3 × 10-6mol L-1. This experiment illustrates how the Kdvalue can be determined and used to evaluate the strength of protein-ligand interactions. This experiment is appropriate for undergraduate students or first-year graduate students in biochemistry, chemistry, or physical chemistry. © 2010 The American Chemical Society and Division of Chemical Education, Inc.