Publication: Effects of chitosan as the growth stimulator for Grammatophyllum speciosum in vitro culture
9
Issued Date
2010-11-01
Resource Type
ISSN
20103778
2010376X
2010376X
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2-s2.0-78651574377
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Mahidol University
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SCOPUS
Bibliographic Citation
World Academy of Science, Engineering and Technology. Vol.71, (2010), 449-451
Suggested Citation
K. Sopalun, K. Thammasiri, K. Ishikawa Effects of chitosan as the growth stimulator for Grammatophyllum speciosum in vitro culture. World Academy of Science, Engineering and Technology. Vol.71, (2010), 449-451. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/29068
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Title
Effects of chitosan as the growth stimulator for Grammatophyllum speciosum in vitro culture
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Abstract
The effects of chitosan, a biodegradable polymer, were studied in Grammatophyllum speciosum protocorm-like bodies (PLBs) in vitro culture. The chitosan concentration of 0, 5, 10, 15, 20, 25, 50 or 100 mg/l were supplemented in half-strength Murashige and Skoog (1/2 MS) liquid or on agar media containing 2% (w/v) sucrose. The results showed that liquid medium supplemented with 15 mg/l chitosan showed the highest relative growth rate (7-fold increase) of PLBs. On 1/2 MS agar medium supplemented with 25 mg/l chitosan gave the highest relative growth rate (4-fold increase). The relative growth rate of G. speciosum PLBs on agar medium was significantly lower than that in liquid medium. Moreover, chitosan, supplemented to agar medium promoted shoot formation but not rooting. However, supplementation at too high a level, such as 100 mg/l can inhibit growth and kill PLBs.