Publication: Copro-DNA diagnosis of Opisthorchis viverrini and Haplorchis taichui infection in an endemic area of LAO PDR
2
Issued Date
2010-01-01
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ISSN
01251562
Other identifier(s)
2-s2.0-76949089139
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.41, No.1 (2010), 28-35
Suggested Citation
Megumi Sato, Tiengkham Pongvongsa, Surapol Sanguankiat, Tipparayat Yoonuan, Paron Dekumyoy, Thareerat Kalambaheti, Malaythong Keomoungkhoun, Inthava Phimmayoi, Boungnong Boupha, Kazuhiko Moji, Jitra Waikagul Copro-DNA diagnosis of Opisthorchis viverrini and Haplorchis taichui infection in an endemic area of LAO PDR. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.41, No.1 (2010), 28-35. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/29855
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Title
Copro-DNA diagnosis of Opisthorchis viverrini and Haplorchis taichui infection in an endemic area of LAO PDR
Abstract
The utility of differential copro-DNA diagnosis using modified sample preparation steps of small liver and minute intestinal fluke infections was tested. Fecal samples containing parasite eggs were washed extensively with diluted detergent solution. Parasite eggs were concentrated by sedimentation and broken by microwaving before DNA extraction. PCR targeting ITS1 and ITS2 regions were performed using primer specific for Opisthorchis viverrini, Haplorchis taichui and other related species. Of 125 fecal samples, 94 were positive for small trematode eggs by a modified cellophane thick smear method. By ITS1-PCR, 52 samples were positive for O. viverrini, 12 H. taichui and 7 mixed infection. By ITS2-PCR, 63 were positive for O. viverini, 17 H. taichui, and 19 mixed infection. The ITS-PCR assay identified a higher number of opisthorchiasis cases than those with O. viverrini expelled after treatment, but for H. taichui, ITS-PCR identified less than half of the worm expelled cases. These results showed that copro-DNA diagnosis was useful for the differential diagnosis of O. viverrini and H. taichui infection, which could not be discriminated by microscopy.