Publication: Sperm capacitation in the fallopian tube of the hamster and its suppression by endocrine factors
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Issued Date
1981-01-01
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ISSN
1097010X
0022104X
0022104X
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2-s2.0-0019618106
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Experimental Zoology. Vol.217, No.3 (1981), 403-407
Suggested Citation
P. Viriyapanich, J. M. Bedford Sperm capacitation in the fallopian tube of the hamster and its suppression by endocrine factors. Journal of Experimental Zoology. Vol.217, No.3 (1981), 403-407. doi:10.1002/jez.1402170311 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/30137
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Title
Sperm capacitation in the fallopian tube of the hamster and its suppression by endocrine factors
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Abstract
Evidence obtained in the rabbit, an induced ovulator, has created a general view that the sperm capacitation ability of the oviduct cannot be modified significantly by ovarian steroids. The possibility that this may not be so in the reproductive tract of cyclic species has been investigated by timed transfer of eggs and spermatozoa into female hamsters maintained in different endocrine states. In estrous females, few native eggs were penetrated by 5 hours, but a majority were penetrated 6–6.5 hours after insemination of fresh epididymal spermatozoa directly into the ovarian bursa. This indicates that a time of approximately 5–6 hours is required for capacitation in the hamster oviduct alone. Up to 2 hours were needed for most eggs to be penetrated after their transfer to oviducts containing fully capacitated spermatozoa. Subsequent experiments using these criteria showed that the ability of the oviduct to support fertilization was affected significantly by the endocrine state of the female. It was not influenced noticeably by ovariectomy. However, whereas maximal levels were seen on days 1,3, and 4, fertilization was reduced significantly (P < 0.001) on day 2 of the estrous cycle when progesterone levels had been elevated for approximately 48 hours. Fertilization of eggs transferred to inseminated oviducts was also reduced comparably in ovariectomized hamsters injected with 3 mg of progesterone twice per day for 9–12 days, and in those that were 8–10 days pregnant. The prospects for complete suppression of capacitation in species with more prolonged cycles and the relevance of this for contraception and analysis of the factors responsible for capacition are discussed. Copyright © 1981 Wiley‐Liss, Inc., A Wiley Company