Publication:
The effect of a ribonuclease inhibitor from human placenta on the in vitro synthesis of human placental proteins

2

Issued Date

1983-02-07

Resource Type

Article

ISSN

00145793

DOI

10.1016/0014-5793(83)80476-1

Other identifier(s)

2-s2.0-0020681664

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

FEBS Letters. Vol.152, No.1 (1983), 35-38

Suggested Citation

W. Hiranyavasit, T. Kusamran The effect of a ribonuclease inhibitor from human placenta on the in vitro synthesis of human placental proteins. FEBS Letters. Vol.152, No.1 (1983), 35-38. doi:10.1016/0014-5793(83)80476-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/30434

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Title

The effect of a ribonuclease inhibitor from human placenta on the in vitro synthesis of human placental proteins

Author(s)

W. Hiranyavasit
 T. Kusamran

Other Contributor(s)

Mahidol University

Abstract

Addition of a ribonuclease inhibitor (10 μg/ml) from human placenta caused 2-3 fold increase of [3H]leucine incorporation in the wheat germ extract as directed by human placental poly (A)-mRNA. Analysis of the translated products by sodiumdodecylsulfate/polyacrylamide gel electrophoresis/fluorography revealed that the inhibitor preferentially increased the yields of the larger proteins, particularly those of larger than Mr40 000. In the presence of the inhibitor, yields of two placental proteins (human placental lactogen and human chorionic gonadotropin) were increased about 70-80% as detected by immunoprecipitation with specific homologous antisera. The method provided an improvement of translation system for studying biosynthesis of other human placental proteins. © 1983.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

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URI

https://repository.li.mahidol.ac.th/handle/123456789/30434

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Scopus 1969-1990