Publication: DNA cloning of plasmodium falciparum circumsporozoite gene: amino acid sequence of repetitive epitope
Issued Date
1984-01-01
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ISSN
00368075
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2-s2.0-0021160478
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Mahidol University
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SCOPUS
Bibliographic Citation
Science. Vol.225, No.4662 (1984), 628-630
Suggested Citation
Vincenzo Enea, Joan Ellis, Fidel Zavala, David E. Arnot, Achara Asavanich, Aoi Masuda, Isabella Quakyi, Ruth S. Nussenzweig DNA cloning of plasmodium falciparum circumsporozoite gene: amino acid sequence of repetitive epitope. Science. Vol.225, No.4662 (1984), 628-630. doi:10.1126/science.6204384 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/30722
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Title
DNA cloning of plasmodium falciparum circumsporozoite gene: amino acid sequence of repetitive epitope
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Abstract
A clone of complementary DNA encoding the circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum has been isolated by screening an Escherichia coli complementary DNA library with a monoclonal antibody to the CS protein. The DNA sequence of the complementary DNA insert encodes a four-amino acid sequence: proline-asparagine-alanine-asparagine, tandemly repeated 23 times. The CS β-lactamase fusion protein specifically binds monoclonal antibodies to the CS protein and inhibits the binding of these antibodies to native Plasmodium falciparum CS protein. These findings provide a basis for the development of a vaccine against Plasmodium falciparum malaria.