Publication: Isolation of organic matrix nacreous proteins from haliotis diversicolor and their effect on in vitro osteoinduction
Submitted Date
Received Date
Accepted Date
Issued Date
2013-12-01
Copyright Date
Announcement No.
Application No.
Patent No.
Valid Date
Resource Type
Edition
Resource Version
Language
File Type
No. of Pages/File Size
ISBN
ISSN
00762997
eISSN
Scopus ID
WOS ID
Pubmed ID
arXiv ID
Call No.
Other identifier(s)
2-s2.0-84892496917
Journal Title
Volume
Issue
item.page.oaire.edition
Start Page
End Page
Access Rights
Access Status
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Physical Location
Bibliographic Citation
Malacologia. Vol.56, No.1-2 (2013), 107-119
Citation
Supita Tanasawet, Boonsirm Withyachumnarnkul, Chinarat Changsangfar, Scott F. Cummins, Morakot Sroyraya, Parisatcha Sangsuwan, Yindee Kitiyanant, Somluk Asuvapongpatana, Wattana Weerachatyanukul (2013). Isolation of organic matrix nacreous proteins from haliotis diversicolor and their effect on in vitro osteoinduction. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/30937.
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Isolation of organic matrix nacreous proteins from haliotis diversicolor and their effect on in vitro osteoinduction
Alternative Title(s)
Author's Affiliation
Author's E-mail
Editor(s)
Editor's Affiliation
Corresponding Author(s)
Creator(s)
Compiler
Advisor(s)
Illustrator(s)
Applicant(s)
Inventor(s)
Issuer
Assignee
Other Contributor(s)
Series
Has Part
Abstract
The organic matrix proteins of molluscan nacre are known to engage biocompatible and osteoinductive properties. In this study, we aimed to extract Haliotis diversicolor nacreous proteins and assayed their effect on pre-osteoblastic cell differentiation. We found that acid extracted nacreous proteins (AEP) consisted primarily of a major protein band of 25 kDa and three other minor proteins. AEP enhanced alkaline phosphatase (ALP) activity of MC3T3-E1 cells both in time-and concentration-dependent manners. Transcriptional up-regulation of osteogenic markers, including collagen type I (COL-I), osteopontin (OPN) and osteocalcin (OCN), was also apparent in days 7 and 14 upon AEP treatments. At the translational level, higher protein expression of COL-I was evident in AEP treated cells, and the protein was presumably laid down as extracellular matrix. Further de novo sequencing of an AEP major protein revealed a match with the abalone mantle protein sometsuke. Conclusively, we demonstrated that H. diversicolor AEP contains a factor, potentially the mantle protein sometsuke, which may impart in the osteoblastic cell differentiation.