Publication: Molecular cloning and functional characterization of Argonaute-3 gene from Penaeus monodon
Issued Date
2013-09-01
Resource Type
ISSN
10959947
10504648
10504648
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2-s2.0-84882827893
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Mahidol University
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SCOPUS
Bibliographic Citation
Fish and Shellfish Immunology. Vol.35, No.3 (2013), 874-882
Suggested Citation
Amnat Phetrungnapha, Teerapong Ho, Apinunt Udomkit, Sakol Panyim, Chalermporn Ongvarrasopone Molecular cloning and functional characterization of Argonaute-3 gene from Penaeus monodon. Fish and Shellfish Immunology. Vol.35, No.3 (2013), 874-882. doi:10.1016/j.fsi.2013.06.025 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/30980
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Title
Molecular cloning and functional characterization of Argonaute-3 gene from Penaeus monodon
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Abstract
Argonaute (Ago) proteins play a crucial role in the shrimp RNA interference pathway. In this study, we identified and characterized a novel Ago gene from black tiger shrimp, Penaeus monodon. The complete open reading frame of P. monodon Ago3. (PmAgo3) consisted of 2559 nucleotides encoding a polypeptide of 852 amino acids with a predicted molecular weight of 97kDa and an isoelectric point of 9.42. Analysis of the deduced amino acid sequence of PmAgo3 revealed the presence of two signature domains of the proteins in Argonaute family including PAZ and PIWI. Phylogenetic analysis indicated that PmAgo3 is classified into Ago subfamily and shared the highest amino acid sequence identity (83%) with Litopenaeus vannamei Ago2. Monitoring of the PmAgo3 expression by quantitative real-time PCR revealed that this gene was significantly up-regulated following dsRNA administration, while no significant difference in its expression was observed following yellow head virus (YHV) challenge. In contrast, inhibition of YHV mRNA expression was observed in PmAgo3-knockdown shrimp. These data imply that PmAgo3 is involved in the dsRNA-mediated gene silencing mechanism and plays an important role in YHV replication in the black tiger shrimp. © 2013 Elsevier Ltd.