Publication:
Development of microsatellite DNA primers for the giant freshwater stingray, Himantura chaophraya (Batoidea: Dasyatidae) in Thailand, and cross-species amplification in other stingrays

Issued Date

2013-05-03

Resource Type

Article

ISSN

18777260
18777252

DOI

10.1007/s12686-012-9826-z

Other identifier(s)

2-s2.0-84876876185

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Conservation Genetics Resources. Vol.5, No.2 (2013), 453-455

Suggested Citation

T. Bhummakasikara, C. Kongrit, C. Siripunkaw, N. Chansue, J. Khudamrongsawat Development of microsatellite DNA primers for the giant freshwater stingray, Himantura chaophraya (Batoidea: Dasyatidae) in Thailand, and cross-species amplification in other stingrays. Conservation Genetics Resources. Vol.5, No.2 (2013), 453-455. doi:10.1007/s12686-012-9826-z Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31026

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Title

Development of microsatellite DNA primers for the giant freshwater stingray, Himantura chaophraya (Batoidea: Dasyatidae) in Thailand, and cross-species amplification in other stingrays

Author(s)

T. Bhummakasikara
 C. Kongrit
 C. Siripunkaw
 N. Chansue
 J. Khudamrongsawat

Other Contributor(s)

Mahidol University
 Chulalongkorn University

Abstract

The lack of animal legislation and the threats of overfishing and water pollution jeopardise the existence of the giant freshwater stingray (Himantura chaophraya) in Thailand. Since the information of this species is scarce, a genetic study is useful for raising the issue of conservation on this species. Polymorphic microsatellite loci were isolated and characterised. The number of alleles ranged from 2 to 10 alleles per locus with an average of 6. 25 alleles per locus. The observed heterozygosity and expected heterozygosity ranged from 0. 2500 to 0. 7586 and 0. 4129 to 0. 8742, respectively. A significant deviation from Hardy-Weinberg equilibrium was observed at some loci. No linkage disequilibrium was observed. Several loci were successfully amplified in other freshwater stingrays. © 2013 Springer Science+Business Media Dordrecht.

Keyword(s)

Agricultural and Biological Sciences
 Biochemistry, Genetics and Molecular Biology

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URI

https://repository.li.mahidol.ac.th/handle/123456789/31026

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Scopus 2011-2015