Publication: Subpopulations of Staphylococcus aureus Clonal Complex 121 Are Associated with Distinct Clinical Entities
Issued Date
2013-03-07
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19326203
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2-s2.0-84874708204
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Mahidol University
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SCOPUS
Bibliographic Citation
PLoS ONE. Vol.8, No.3 (2013)
Suggested Citation
Kevin Kurt, Jean Philippe Rasigade, Frederic Laurent, Richard V. Goering, Helena Žemličková, Ivana Machova, Marc J. Struelens, Andreas E. Zautner, Silva Holtfreter, Barbara Bröker, Stephen Ritchie, Sin Reaksmey, Direk Limmathurotsakul, Sharon J. Peacock, Christiane Cuny, Franziska Layer, Wolfgang Witte, Ulrich Nübel Subpopulations of Staphylococcus aureus Clonal Complex 121 Are Associated with Distinct Clinical Entities. PLoS ONE. Vol.8, No.3 (2013). doi:10.1371/journal.pone.0058155 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31058
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Title
Subpopulations of Staphylococcus aureus Clonal Complex 121 Are Associated with Distinct Clinical Entities
Author(s)
Kevin Kurt
Jean Philippe Rasigade
Frederic Laurent
Richard V. Goering
Helena Žemličková
Ivana Machova
Marc J. Struelens
Andreas E. Zautner
Silva Holtfreter
Barbara Bröker
Stephen Ritchie
Sin Reaksmey
Direk Limmathurotsakul
Sharon J. Peacock
Christiane Cuny
Franziska Layer
Wolfgang Witte
Ulrich Nübel
Jean Philippe Rasigade
Frederic Laurent
Richard V. Goering
Helena Žemličková
Ivana Machova
Marc J. Struelens
Andreas E. Zautner
Silva Holtfreter
Barbara Bröker
Stephen Ritchie
Sin Reaksmey
Direk Limmathurotsakul
Sharon J. Peacock
Christiane Cuny
Franziska Layer
Wolfgang Witte
Ulrich Nübel
Other Contributor(s)
Robert Koch Institut
Universite de Lyon
Creighton University School of Medicine
National Institute of Public Health Prague
European Centre for Disease Prevention and Control
Universitatsmedizin Gottingen
Ernst-Moritz-Arndt-Universitat Greifswald
University of Auckland
Angkor Hospital for Children
Mahidol University
University of Cambridge
Universite de Lyon
Creighton University School of Medicine
National Institute of Public Health Prague
European Centre for Disease Prevention and Control
Universitatsmedizin Gottingen
Ernst-Moritz-Arndt-Universitat Greifswald
University of Auckland
Angkor Hospital for Children
Mahidol University
University of Cambridge
Abstract
We investigated the population structure of Staphylococcus aureus clonal complex CC121 by mutation discovery at 115 genetic housekeeping loci from each of 154 isolates, sampled on five continents between 1953 and 2009. In addition, we pyro-sequenced the genomes from ten representative isolates. The genome-wide SNPs that were ascertained revealed the evolutionary history of CC121, indicating at least six major clades (A to F) within the clonal complex and dating its most recent common ancestor to the pre-antibiotic era. The toxin gene complement of CC121 isolates was correlated with their SNP-based phylogeny. Moreover, we found a highly significant association of clinical phenotypes with phylogenetic affiliations, which is unusual for S. aureus. All isolates evidently sampled from superficial infections (including staphylococcal scalded skin syndrome, bullous impetigo, exfoliative dermatitis, conjunctivitis) clustered in clade F, which included the European epidemic fusidic-acid resistant impetigo clone (EEFIC). In comparison, isolates from deep-seated infections (abscess, furuncle, pyomyositis, necrotizing pneumonia) were disseminated in several clades, but not in clade F. Our results demonstrate that phylogenetic lineages with distinct clinical properties exist within an S. aureus clonal complex, and that SNPs serve as powerful discriminatory markers, able to identify these lineages. All CC121 genomes harboured a 41-kilobase prophage that was dissimilar to S. aureus phages sequenced previously. Community-associated MRSA and MSSA from Cambodia were extremely closely related, suggesting this MRSA arose in the region. © 2013 Kurt et al.