Publication: Molecular and functional characterization of a dopamine receptor type1 from Penaeus monodon
Issued Date
2013-03-04
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ISSN
00448486
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2-s2.0-84871887209
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Mahidol University
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SCOPUS
Bibliographic Citation
Aquaculture. Vol.380-383, (2013), 99-105
Suggested Citation
Suchitraporn Sukthaworn, Sakol Panyim, Apinunt Udomkit Molecular and functional characterization of a dopamine receptor type1 from Penaeus monodon. Aquaculture. Vol.380-383, (2013), 99-105. doi:10.1016/j.aquaculture.2012.12.001 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31059
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Title
Molecular and functional characterization of a dopamine receptor type1 from Penaeus monodon
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Abstract
Dopamine (DA) is a biogenic amine neurotransmitter that is involved in the regulation of gonadal maturation in crustacean, presumably by inhibiting the stimulatory effect of serotonin on gonad development. Like other neurotransmitters, DA exerts its effect through a membrane-bound G protein-coupled receptor. In this study, a cDNA encoding DA receptor of Penaeus monodon, so called PemDopR1, was obtained by Rapid Amplification of cDNA Ends (RACE) strategy. The PemDopR1 cDNA was 1990 nucleotides long harboring a 1356bp open reading frame that encodes a putative PemDopR1 protein of 452 amino acids. Analysis of the deduced amino acid sequence of PemDopR1 revealed seven transmembrane domains and other common signatures of G protein-coupled receptors. PemDopR1 is clustered with type 1 of DA receptor with the highest similarity to D1βof the spiny lobster. Functional assay of a recombinant PemDopR1 in COS1-cells showed an increase in cAMP level upon DA activation confirming that PemDopR1 belongs to type 1 DA receptor. The mRNA of PemDopR1 was highly expressed in the central nervous system, especially in the brain. The expression of PemDopR1 in the brain throughout ovarian maturation cycle revealed a decreased expression in the maturation stage. Our results suggested that the inhibitory effect of DA during the early stages of ovarian maturation is mediated via PemDopR1. © 2012 Elsevier B.V.