Publication: Molecular and histological identification of Marteilioides infection in suminoe Oyster Crassostrea ariakensis, manila clam Ruditapes philippinarum and pacific Oyster Crassostrea gigas on the south coast of Korea
Issued Date
2013-01-01
Resource Type
ISSN
10960805
00222011
00222011
Other identifier(s)
2-s2.0-84884661569
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Invertebrate Pathology. Vol.114, No.3 (2013), 277-284
Suggested Citation
Limpanont Yanin, Hyun Sil Kang, Hyun Ki Hong, Hee Do Jeung, Bong Kyu Kim, Thanh Cuong Le, Young Ok Kim, Kwang Sik Choi Molecular and histological identification of Marteilioides infection in suminoe Oyster Crassostrea ariakensis, manila clam Ruditapes philippinarum and pacific Oyster Crassostrea gigas on the south coast of Korea. Journal of Invertebrate Pathology. Vol.114, No.3 (2013), 277-284. doi:10.1016/j.jip.2013.08.008 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31103
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Title
Molecular and histological identification of Marteilioides infection in suminoe Oyster Crassostrea ariakensis, manila clam Ruditapes philippinarum and pacific Oyster Crassostrea gigas on the south coast of Korea
Abstract
The oyster ovarian parasite Marteilioides chungmuensis has been reported from Korea and Japan, damaging the oyster industries. Recently, Marteilioides-like organisms have been identified in other commercially important marine bivalves. In this study, we surveyed Marteilioides infection in the Manila clam Ruditapes philippinarum, Suminoe oyster Crassostrea ariakensis, and Pacific oyster Crassostrea gigas, using histology and Marteilioides-specific small subunit (SSU) rDNA PCR. The SSU rDNA sequence of M. chungmuensis (1716 bp) isolated from C. gigas in Tongyoung bay was 99.9% similar to that of M. chungmuensis reported in Japan. Inclusions of multi-nucleated bodies in the oocytes, typical of Marteilioides infection, were identified for the first time in Suminoe oysters. The SSU rDNA sequence of a Marteilioides-like organism isolated from Suminoe oysters was 99.9% similar to that of M. chungmuensis. Marteilioides sp. was also observed from 7 Manila clams of 1840 individuals examined, and the DNA sequences of which were 98.2% similar to the known sequence of M. chungmuensis. Unlike Marteilioides infection of Pacific oysters, no remarkable pathological symptoms, such as large multiple lumps on the mantle, were observed in infected Suminoe oysters or Manila clams. Distribution of the infected Manila clams, Suminoe oysters and Pacific oysters was limited to small bays on the south coast, suggesting that the southern coast is the enzootic area of Marteilioides infection. © 2013 Elsevier Inc.