Publication: Production of Japanese encephalitis virus-like particles in insect cells
Issued Date
2013-11-19
Resource Type
ISSN
19491026
19491018
19491018
Other identifier(s)
2-s2.0-84887539712
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Mahidol University
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SCOPUS
Bibliographic Citation
Bioengineered. Vol.4, No.6 (2013)
Suggested Citation
Hideki Yamaji, Eiji Konishi Production of Japanese encephalitis virus-like particles in insect cells. Bioengineered. Vol.4, No.6 (2013). doi:10.4161/bioe.24514 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31163
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Title
Production of Japanese encephalitis virus-like particles in insect cells
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Abstract
Virus-like particles (VLPs) are composed of one or several recombinant viral surface proteins that spontaneously assemble into particulate structures without the incorporation of virus DNA or rna. The baculovirus-insect cell system has been used extensively for the production of recombinant virus proteins including VLPs. While the baculovirus-insect cell system directs the transient expression of recombinant proteins in a batch culture, stably transformed insect cells allow constitutive production. in our recent study, a secretory form of Japanese encephalitis (Je) VLPs was successfully produced by Trichoplusia ni BTi-Tn-5B1-4 (high Five) cells engineered to coexpress the Je virus (JEV) premembrane (PRM) and envelope (e) proteins. a higher yield of e protein was attained with recombinant high Five cells than with the baculovirus-insect cell system. This study demonstrated that recombinant insect cells offer a promising approach to the high-level production of VLPs for use as vaccines and diagnostic antigens. © 2013 Landes Bioscience.