Publication:
Potential of capillary electrophoresis (CE) and chip-CE with dual detection (capacitively-coupled contactless conductivity detection (C<inf>4</inf>D) and fluorescence detection) for monitoring of nicotine and cotinine derivatization

2

Issued Date

2013-04-03

Resource Type

Article

ISSN

13482246
09106340

DOI

10.2116/analsci.29.339

Other identifier(s)

2-s2.0-84875553504

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Analytical Sciences. Vol.29, No.3 (2013), 339-344

Suggested Citation

Nantana Nuchtavorn, Marketa Ryvolova, Fritz Bek, Mirek Macka, Chutima Phechkrajang, Leena Suntornsuk Potential of capillary electrophoresis (CE) and chip-CE with dual detection (capacitively-coupled contactless conductivity detection (C<inf>4</inf>D) and fluorescence detection) for monitoring of nicotine and cotinine derivatization. Analytical Sciences. Vol.29, No.3 (2013), 339-344. doi:10.2116/analsci.29.339 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31543

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Title

Potential of capillary electrophoresis (CE) and chip-CE with dual detection (capacitively-coupled contactless conductivity detection (C<inf>4</inf>D) and fluorescence detection) for monitoring of nicotine and cotinine derivatization

Author(s)

Nantana Nuchtavorn
 Marketa Ryvolova
 Fritz Bek
 Mirek Macka
 Chutima Phechkrajang
 Leena Suntornsuk

Other Contributor(s)

Siam University
 Mahidol University
 National Centre for Sensor Research, Dublin
 Agilent Technologies Deutschland GmbH
 University of Tasmania

Abstract

Capillary electrophoresis (CE) coupled with capacitively-coupled contactless conductivity (C4D) and fluorescence (FD) detectors and chip-CE for monitoring of nicotine and cotinine derivatization was demonstrated. Separation of the substrates and intermediates could be achieved by CE-C4D in 7 min (Rs > 2.7) using 45 mM acetic acid (pH 3.0) and this system was applied to detect the intermediate formation. Final fluorescent products could be analyzed by micellar electrokinetic chromatography (MEKC-FD) in 5 mM borate buffer (pH 9.0) containing 10 mM sodium dodecylsulfate (SDS) (%RSD < 3.00%). Transferring of MEKC-FD to chip-CE allowed for shorter analysis time (2.5 min) and decreased sample consumption. The chip-CE-FD shows good detection and quantitation limits (< 7.5 μM) and precision (%RSD < 2.81%) and was employed to determine nicotine and cotinine in artificial urine. This work reveals the potential of CE and chip-CE with dual detectors as simultaneous, convenient and rapid methods for monitoring pyridine derivatization. © The Japan Society for Analytical Chemistry.

Keyword(s)

Chemistry

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https://repository.li.mahidol.ac.th/handle/123456789/31543

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