Publication: Rapid detection and identification of Brugia malayi, B. pahangi, and dirofilaria immitis by high-resolution melting assay
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Issued Date
2013-01-01
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ISSN
15577759
15303667
15303667
Other identifier(s)
2-s2.0-84872181098
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Mahidol University
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SCOPUS
Bibliographic Citation
Vector-Borne and Zoonotic Diseases. Vol.13, No.1 (2013), 31-36
Suggested Citation
Sirichit Wongkamchai, Nuntiya Monkong, Pakpimom Mahannol, Piyanan Taweethavonsawat, Sumat Loymak, Suporn Foongladda Rapid detection and identification of Brugia malayi, B. pahangi, and dirofilaria immitis by high-resolution melting assay. Vector-Borne and Zoonotic Diseases. Vol.13, No.1 (2013), 31-36. doi:10.1089/vbz.2012.0971 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31989
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Title
Rapid detection and identification of Brugia malayi, B. pahangi, and dirofilaria immitis by high-resolution melting assay
Abstract
Human lymphatic filariasis is caused by filarial worms such as Brugia malayi for which the major reservoir is domestic cats. However, domestic cats or dogs also carry nonhuman filaria such as Brugia pahangi and Dirofilaria immitis. We have developed a single-tube, real-time PCR with a high-resolution melting (HRM) analysis assay for detection and identification of B. malayi, B. pahangi, and D. immitis in blood samples. The designated primer pair in the PCR can amplify a 114-bp region of mitochondrial 12S rRNA genes of these filarial worms. Subsequently, the HRM assay showed a specific melting temperature for each species. The assay showed the highest sensitivity and specificity in comparison with DNA sequences after assessment with 34 cat and 14 dog blood samples. This assay could be helpful for epidemiological studies of reservoirs and vectors. © Copyright 2013, Mary Ann Liebert, Inc. 2013.