Publication: The role of short-chain dehydrogenase/ oxidoreductase, induced by salt stress, on host interaction of B. pseudomallei
Issued Date
2014
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eng
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Mahidol University
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BioMed Central
Bibliographic Citation
BMC Microbiology. Vol.14, (2014), 1
Suggested Citation
Pornpan Pumirat, Usa Boonyuen, Muthita Vanaporn, Peechanika Pinweha, Sarunporn Tandhavanant, Sunee Korbsrisate, Narisara Chantratita The role of short-chain dehydrogenase/ oxidoreductase, induced by salt stress, on host interaction of B. pseudomallei. BMC Microbiology. Vol.14, (2014), 1. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/3225
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Title
The role of short-chain dehydrogenase/ oxidoreductase, induced by salt stress, on host interaction of B. pseudomallei
Abstract
Background: Burkholderia pseudomallei is the causative agent of melioidosis, a frequently occurring disease in
northeastern Thailand, where soil and water high in salt content are common. Using microarray analysis, we
previously showed that B. pseudomallei up-regulated a short-chain dehydrogenase/oxidoreductase (SDO) under salt
stress. However, the importance of SDO in B. pseudomallei infection is unknown. This study aimed to explore the
function of B. pseudomallei SDO, and to investigate its role in interactions between B. pseudomallei and host cells.
Results: Bioinformatics analysis of B. pseudomallei SDO structure, based on homology modeling, revealed a NAD+
cofactor domain and a catalytic triad containing Ser149, Tyr162, and Lys166. This is similar to Bacillus megaterium
glucose 1-dehydrogenase. To investigate the role of this protein, we constructed a B. pseudomallei SDO defective
mutant, measured glucose dehydrogenase (GDH) activity, and tested the interactions with host cells. The B. pseudomallei
K96243 wild type exhibited potent GDH activity under condition containing 300 mM NaCl, while the mutant showed
activity levels 15 times lower. Both invasion into the A549 cell line and early intracellular survival within the J774A.1
macrophage cell were impaired in the mutant. Complementation of SDO was able to restore the mutant ability to
produce GDH activity, invade epithelial cells, and survive in macrophages.
Conclusions: Our data suggest that induced SDO activity during salt stress may facilitate B. pseudomallei invasion
and affect initiation of successful intracellular infection. Identifying the role of B. pseudomallei SDO provides a better
understanding of the association between bacterial adaptation and pathogenesis in melioidosis.