Publication: Cloning and application of recombinant dengue virus prM-M protein for serodiagnosis of dengue virus infection
1
Issued Date
2013-05-22
Resource Type
ISSN
01251562
Other identifier(s)
2-s2.0-84877845091
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.44, No.2 (2013), 218
Suggested Citation
Sineewanlaya Wichit, Hatairat Yongpradoem, Pornapat Surasombatpattana, Pornsawan Leaungwuttiwong, Thareerat Kalambaheti, Wipawee Jampangern, Akanitt Jittmittraphap Cloning and application of recombinant dengue virus prM-M protein for serodiagnosis of dengue virus infection. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.44, No.2 (2013), 218. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/32339
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Cloning and application of recombinant dengue virus prM-M protein for serodiagnosis of dengue virus infection
Other Contributor(s)
Abstract
We studied the use of the precursor to the M structural protein (prM) found only on the surface of mature dengue virus as a target protein to detect dengue virus infection. Recombinant D2-16681 prM-M protein was constructed and tested for immunogenicity with dengue and Japanese encephalitis patient sera by Western blot analysis and indirect ELISA. The sensitivity and specificity of indirect ELISA were 48.1 and 85.5%, respectively, and Western blot assay were 23.1 and 98.7%, respectively, for detection of dengue virus. Although the sensitivity of the indirect ELISA is low, the indirect ELISA using recombinant D2-16681 prM-M proteins as antigen may be used for early detection of dengue virus infection.