Publication: Molecular mechanism of anti-inflammatory activity of Pluchea indica leaves in macrophages RAW 264.7 and its action in animal models ofinflammation
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Issued Date
2013-03-27
Resource Type
ISSN
18727573
03788741
03788741
Other identifier(s)
2-s2.0-84875208836
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Ethnopharmacology. Vol.146, No.2 (2013), 495-504
Suggested Citation
Doungnapa Buapool, Nadtaya Mongkol, Jirapa Chantimal, Sittiruk Roytrakul, Ekaruth Srisook, Kloakwan Srisook Molecular mechanism of anti-inflammatory activity of Pluchea indica leaves in macrophages RAW 264.7 and its action in animal models ofinflammation. Journal of Ethnopharmacology. Vol.146, No.2 (2013), 495-504. doi:10.1016/j.jep.2013.01.014 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/32745
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Title
Molecular mechanism of anti-inflammatory activity of Pluchea indica leaves in macrophages RAW 264.7 and its action in animal models ofinflammation
Abstract
Ethnopharmacological relevance: Pluchea indica Less.: (Asteraceae) is a Thai medicinal plant used in traditional medicine for the treatment of hemorrhoids, lumbago, leucorrhoea and inflammation. This study investigated the molecular mechanism of anti-inflammatory activity of Pluchea indica leaf extract in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and also determined its action in acute inflammation animal models. Materials and methods: The inhibitory effect of Pluchea indica leaf extract on LPS-induced nitric oxide (NO) production was evaluated by Griess reaction. Protein and mRNA expressions were determined by real time RT-PCR and Western blotting analysis, respectively. Inducible nitric oxide synthase (iNOS) promoter activity was evaluated by iNOS promoter based reporter gene assay. In vivo anti-inflammatory effect was examined in ethylphenylpropiolate (EPP)-induced ear edema and carrageenan-induced paw edema in rat models. Results: Ethyl acetate fraction of ethanol extract of Pluchea indica leaves (EFPI) exhibited the potent inhibitory effect on NO production in LPS-induced macrophages and also inhibited PGE2release. EFPI reduced iNOS mRNA and protein expression through suppressed iNOS promoter activity and nuclear translocation of subunit p65 of nuclear factor-κB, but did not inhibit phosphorylation of the mitogen-activated protein kinases (MAPKs). Moreover, EFPI possessed anti-inflammatory activities on acute phase of inflammation as seen in EPP-induced ear edema and carrageenan-induced paw edema inrats. Conclusions: These data support the pharmacological basis of Pluchea indica plant as a traditional herbal medicine for treatment of inflammation. © 2013 Elsevier Ireland Ltd.