Publication: Vitellogenin gene expression at different ovarian stages in the giant freshwater prawn, Macrobrachium rosenbergii, and stimulation by 4-nonylphenol
9
Issued Date
2014-01-01
Resource Type
ISSN
13652109
1355557X
1355557X
Other identifier(s)
2-s2.0-84892436325
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Aquaculture Research. Vol.45, No.2 (2014), 320-326
Suggested Citation
Ferdause Ara, Praneet Damrongphol Vitellogenin gene expression at different ovarian stages in the giant freshwater prawn, Macrobrachium rosenbergii, and stimulation by 4-nonylphenol. Aquaculture Research. Vol.45, No.2 (2014), 320-326. doi:10.1111/j.1365-2109.2012.03229.x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/33071
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Vitellogenin gene expression at different ovarian stages in the giant freshwater prawn, Macrobrachium rosenbergii, and stimulation by 4-nonylphenol
Author(s)
Other Contributor(s)
Abstract
Vitellogenin gene (Vg) expression was investigated in the giant freshwater prawn, Macrobrachium rosenbergii at different ovarian stages and after exposure to 4-nonylphenol (4-NP), a widespread xeno-oestrogen present in aquatic environments. Reverse transcriptase polymerase chain reaction (RT-PCR) revealed a high level of Vg expression in the hepatopancreas at all ovarian stages, but at a much lower level in the ovary and real-time PCR showed differential Vg expression in both the hepatopancreas and ovary. Vg expression in the hepatopancreas increased significantly at the mature ovarian stage. The expression profile in the ovary was the inverse of that in the hepatopancreas. Furthermore, the hepatopancreas was more responsive to the oestrogenic activity of 4-NP. Exposure to 50-250 μg L-1 4-NP for 96 h increased Vg expression in the hepatopancreas in a dose-dependent manner, whereas Vg expression was less in the ovary. In conclusion, Vg expression was tissue- and stage-dependent. These factors must be considered when Vg expression is used as an indicator of reproductive activity in an organism or for assessing xeno-oestrogens in the environments. © 2012 John Wiley & Sons Ltd.