Publication: T-DNA insertion alters the terpenoid content composition and bioactivity of transgenic Artemisia annua
4
Issued Date
2014-01-01
Resource Type
ISSN
15559475
1934578X
1934578X
Other identifier(s)
2-s2.0-84895494903
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Natural Product Communications. Vol.9, No.3 (2014), 363-366
Suggested Citation
Netiya Karaket, Suthep Wiyakrutta, Marie Aleth Lacaille-Dubois, Kanyaratt Supaibulwatana T-DNA insertion alters the terpenoid content composition and bioactivity of transgenic Artemisia annua. Natural Product Communications. Vol.9, No.3 (2014), 363-366. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/33126
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
T-DNA insertion alters the terpenoid content composition and bioactivity of transgenic Artemisia annua
Other Contributor(s)
Abstract
In this study, the interference of T-DNA insertion upon Agrobacterium-mediated transformation on the biochemical expression of the host genome is discussed. Plant extracts of transgenic Artemisia annua L. with or without an overexpressed farnesyl pyrophosphate synthase gene have been investigated for their bioactivity and metabolic profile in comparison with wild type A. annua. The highest antimicrobial activity against Staphylococcus aureus, Bacillus subtilis and Candida albicans was observed in the T253 transgenic lines. Moreover, the crude extract from T253 showed higher antimalarial activity against the Plasmodium faciparum K1 strain than those of the others. The terpenoid constituents and antimicrobial properties of the plant samples were grouped by hierarchical clustering analysis. The clustering showed that squalene is a putative compound that might be involved in increasing the bioactivity of the transgenic line. In addition, T253 had a triterpene content that was about twice as great as that of the T253-2 line, which had a higher content of sesquiterpenes. However, both lines were transformed by the same FPS gene. These results suggested that the different bioactive properties observed in each transgenic line may be caused by variations in their terpenoid composition, which is affected by T-DNA insertion at different positions in the host plant.