Publication: Alterations in nanoparticle protein corona by biological surfactants: Impact of bile salts on β-lactoglobulin-coated gold nanoparticles
Issued Date
2014-07-15
Resource Type
ISSN
10957103
00219797
00219797
Other identifier(s)
2-s2.0-84899789013
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Colloid and Interface Science. Vol.426, (2014), 333-340
Suggested Citation
Thunnalin Winuprasith, Sirinya Chantarak, Manop Suphantharika, Lili He, David Julian McClements Alterations in nanoparticle protein corona by biological surfactants: Impact of bile salts on β-lactoglobulin-coated gold nanoparticles. Journal of Colloid and Interface Science. Vol.426, (2014), 333-340. doi:10.1016/j.jcis.2014.04.018 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/33564
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Title
Alterations in nanoparticle protein corona by biological surfactants: Impact of bile salts on β-lactoglobulin-coated gold nanoparticles
Abstract
The impact of biological surfactants (bile salts) on the protein (β-lactoglobulin) corona surrounding gold nanoparticles (200. nm) was studied using a variety of analytical techniques at pH 7: dynamic light scattering (DLS); particle electrophoresis (ζ-potential); UV-visible (UV) spectroscopy transmission electron microscopy (TEM); and surface-enhanced Raman scattering (SERS). The bile salts adsorbed to the protein-coated nanoparticle surfaces and altered their interfacial composition, charge, and structure. SERS spectra of protein-coated nanoparticles after bile salt addition contained bands from both protein and bile salts, indicating that the protein was not fully displaced by the bile salts. UV, DLS and TEM techniques also indicated that the protein coating was not fully displaced from the nanoparticle surfaces. The impact of bile salts could be described by an orogenic mechanism: mixed interfaces were formed that consisted of islands of aggregated proteins surrounded by a sea of bile salts. This knowledge is useful for understanding the interactions of bile salts with protein-coated colloidal particles, which may be important for controlling the fate of colloidal delivery systems in the human gastrointestinal tract, or the gastrointestinal fate of ingested inorganic nanoparticles. © 2014 Elsevier Inc.