Publication: Short report: Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei
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Issued Date
2014-01-01
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ISSN
00029637
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2-s2.0-84902279551
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.90, No.6 (2014), 1043-1046
Suggested Citation
Brea D. Duval, Mindy G. Elrod, Jay E. Gee, Narisara Chantratita, Sarunporn Tandhavanant, Direk Limmathurotsakul, Alex R. Hoffmaster Short report: Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei. American Journal of Tropical Medicine and Hygiene. Vol.90, No.6 (2014), 1043-1046. doi:10.4269/ajtmh.14-0025 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/34031
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Title
Short report: Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei
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Abstract
Cases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia mallei, respectively) are classified as Tier 1 select agents because of concerns about their potential use as bioterrorism agents. A rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. Our laboratory has further evaluated a latex agglutination assay for its ability to identify B. pseudomallei and B. mallei isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysaccharide produced by B. pseudomallei and B. mallei, but is absent in closely related Burkholderia species. A total of 110 B. pseudomallei and B. mallei were tested, and 36 closely related Burkholderia species. The latex agglutination assay was positive for 109 of 110 (99.1% sensitivity) B. pseudomallei and B. mallei isolates tested. Copyright © 2014 by The American Society of Tropical Medicine and Hygiene.