Publication: The effects of feeding β-glucan to Pangasianodon hypophthalmus on immune gene expression and resistance to Edwardsiella ictaluri
1
Issued Date
2015-11-01
Resource Type
ISSN
10959947
10504648
10504648
Other identifier(s)
2-s2.0-84944075394
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Fish and Shellfish Immunology. Vol.47, No.1 (2015), 595-605
Suggested Citation
Wanna Sirimanapong, Kim D. Thompson, Ei Lin Ooi, Michaël Bekaert, Bertrand Collet, John B. Taggart, James E. Bron, Darren M. Green, Andrew P. Shinn, Alexandra Adams, Michael J. Leaver The effects of feeding β-glucan to Pangasianodon hypophthalmus on immune gene expression and resistance to Edwardsiella ictaluri. Fish and Shellfish Immunology. Vol.47, No.1 (2015), 595-605. doi:10.1016/j.fsi.2015.09.042 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/35083
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
The effects of feeding β-glucan to Pangasianodon hypophthalmus on immune gene expression and resistance to Edwardsiella ictaluri
Abstract
© 2015 Elsevier Ltd. Pangasianodon hypophthalmus (striped catfish) is an important aquaculture species and intensification of farming has increased disease problems, particularly Edwardsiella ictaluri. The effects of feeding β-glucans on immune gene expression and resistance to E. ictaluri in P. hypophthalmus were explored. Fish were fed 0.1% fungal-derived β-glucan or 0.1% commercial yeast-derived β-glucan or a basal control diet without glucan. After 14 days of feeding, the mRNA expression of immune genes (transferrin, C-reactive protein, precerebellin-like protein, Complement C3 and factor B, 2a MHC class II and interleukin-1beta) in liver, kidney and spleen were determined. Following this fish from each of the three diet treatment groups were infected with E. ictaluri and further gene expression measured 24 h post-infection (h.p.i.), while the remaining fish were monitored over 2 weeks for mortalities. Cumulative percentage mortality at 14 days post-infection (d.p.i.) was less in β-glucan fed fish compared to controls. There was no difference in gene expression between dietary groups after feeding for 14 days, but there was a clear difference between infected and uninfected fish at 24 h.p.i., and based on principal component analysis β-glucans stimulated the overall expression of immune genes in the liver, kidney and spleen at 24 h.p.i.