Publication: Polyphenolic compounds and antioxidant activities of the leaves of glochidion hypoleucum
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Issued Date
2015-01-01
Resource Type
ISSN
15559475
1934578X
1934578X
Other identifier(s)
2-s2.0-84930973043
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Mahidol University
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SCOPUS
Bibliographic Citation
Natural Product Communications. Vol.10, No.3 (2015), 479-482
Suggested Citation
Natthinee Anantachoke, Worawan Kitphati, Supachoke Mangmool, Nuntavan Bunyapraphatsara Polyphenolic compounds and antioxidant activities of the leaves of glochidion hypoleucum. Natural Product Communications. Vol.10, No.3 (2015), 479-482. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/35254
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Title
Polyphenolic compounds and antioxidant activities of the leaves of glochidion hypoleucum
Abstract
Bioassay-guided fractionation of the methanol extract of Glochidion hypoleucum (Miq.) Boerl leaves led to the isolation of five polyphenolic compounds, methyl gallate, gallic acid, apigenin-8-Cβ]-D-glucopyranoside (vitexin), luteolin-8-Cβ]-D-glucopyranoside (orientin), and luteolin-6-Cβ]-D-glucopyranoside (isoorientin). The chemical structures of the isolated compounds were determined using spectroscopic (NMR, UV-Vis, IR) and mass spectrometric techniques. The antioxidative properties of the methanol extract and isolated polyphenols were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) for radical scavenging activity and 2.,S,7.,S-dichlorodihydrofluorescein diacetate (DCFH-DA) to measure the level of reactive oxygen species (ROS). With the exception of vitexin, the crude methanol extract and the polyphenolic compounds inhibited DPPH radicals with IC50 values ranging from 2.46 ± 0.05 to 40.0 ± 0.3 μg/mL. In addition, the crude methanol extract attenuated H2O2-induced intracellular ROS production in a dose-dependent manner in HEK-293 cells. Gallic acid and isoorientin significantly reduced the ROS level in HEK-293 cells at a concentration of 20 μM.