Publication: Comparison of cryopreserved human sperm between ultra rapid freezing and slow programmable freezing: Effect on motility, morphology and DNA integrity
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Issued Date
2015-01-01
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01252208
01252208
01252208
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2-s2.0-84938080233
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol.98, (2015), S33-S42
Suggested Citation
Pattama Tongdee, Matchuporn Sukprasert, Chonticha Satirapod, Anna Wongkularb, Wicharn Choktanasiri Comparison of cryopreserved human sperm between ultra rapid freezing and slow programmable freezing: Effect on motility, morphology and DNA integrity. Journal of the Medical Association of Thailand. Vol.98, (2015), S33-S42. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/36829
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Title
Comparison of cryopreserved human sperm between ultra rapid freezing and slow programmable freezing: Effect on motility, morphology and DNA integrity
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Abstract
© 2015, Medical Association of Thailand. All rights reserved. Background: Cryopreservation of sperm is common methods to preserve male fertility. Sperm freezing, suggest slow programmable freezing caused lower change of sperm morphology than sperm freezing in vapor of liquid nitrogen. Ultra rapid freezing is easy to be worked on, less time, low cost and does not need high experience. Objective: To compare the effect on sperm motility, morphology and DNA integrity of post-thawed sperm after ultra rapid freezing and slow programmable freezing methods. Material and Method: Experimental study at laboratory of infertility unit, Department of Obstetrics and Gynecology, Faculty of Medicine Ramathibodi Hospital. Thirty-seven semen samples with normal semen analysis according to World Health Organization (WHO) 1999 [normal sperm volume (≥2 ml) and normal sperm concentration (≥20×10<sup>6</sup>/ml) and sperm motility (≥50%)]. Semen samples were washed. Then each semen sample was divided into six cryovials. Two cryovials, 0.5 ml each, were cryopreserved by slow programmable freezing. Four 0.25 ml containing cryovials, were cryopreserved by ultra rapid freezing method. After cryopreservation for 1 month, thawed process was carried out at room temperature. Main outcomes are sperm motility was determined by Computer-Assisted Semen Analysis (CASA), sperm morphology was determined by eosin-methylene blue staining and sperm DNA integrity was assessed by TUNEL assay. Results: Sperm motility was reduced significantly by both methods, from 70.4 (9.0)% to 29.1 (12.3)% in slow programmable freezing and to 19.7 (9.8)% in ultra rapid freezing (p<0.05). Sperm motility decreased significantly more by ultra rapid freezing (p<0.001). The percentage of normal sperm morphology and DNA integrity were also reduced significantly by both methods. However, no significant difference between the two methods was found (p>0.05). Conclusion: Cryopreservation of human sperm for 1 month significantly decreased sperm motility, morphology and DNA integrity in both methods. However, sperm motility was decreased more by ultra rapid freezing.