Publication: In vitro study of anti-coccidial activity of essential oils from indigenous plants against Eimeria tenella
Submitted Date
Received Date
Accepted Date
Issued Date
2016-09-15
Copyright Date
Announcement No.
Application No.
Patent No.
Valid Date
Resource Type
Edition
Resource Version
Language
File Type
No. of Pages/File Size
ISBN
ISSN
18732550
03044017
03044017
eISSN
Scopus ID
WOS ID
Pubmed ID
arXiv ID
Call No.
Other identifier(s)
2-s2.0-85006745173
Journal Title
Volume
Issue
item.page.oaire.edition
Start Page
End Page
Access Rights
Access Status
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Physical Location
Bibliographic Citation
Veterinary Parasitology. Vol.228, (2016), 96-102
Citation
Surapan Jitviriyanon, Phanida Phanthong, Pattamapan Lomarat, Nuntavan Bunyapraphatsara, Sarthorn Porntrakulpipat, Nuanchan Paraksa (2016). In vitro study of anti-coccidial activity of essential oils from indigenous plants against Eimeria tenella. Retrieved from: https://hdl.handle.net/20.500.14594/40709.
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
In vitro study of anti-coccidial activity of essential oils from indigenous plants against Eimeria tenella
Alternative Title(s)
Author's Affiliation
Author's E-mail
Editor(s)
Editor's Affiliation
Corresponding Author(s)
Creator(s)
Compiler
Advisor(s)
Illustrator(s)
Applicant(s)
Inventor(s)
Issuer
Assignee
Series
Has Part
Abstract
© 2016 Elsevier B.V. This study was designed to evaluate the in vitro anticoccidial properties against Eimeria tenella of different essential oils and their major active components. Efficacy of ten essential oils from different Thai indigenous plants were preliminarily screened and only those with potential were further tested for effective concentrations and identifying their active compounds. Oocysticidal property was evaluated in term of sporulation inhibition of oocysts and the percentage of unsporulated, sporulated and degenerated oocysts, after treatment with 125 μg/ml of the selected essential oil, the sample was enumerated by haemocytometer, while coccidiocidal activity was assessed by the inhibition of sporozoite invasion in MDBK cell lines. Results showed that only Boesenbergia pandurata and Ocimum basilicum essential oils had strong sporulation inhibition activity by providing a higher ratio of degenerated oocysts and their IC50 were 0.134 and 0.101 mg/ml, respectively. GC–MS analysis of B. pandurata essential oil found trans-b-ocimene, camphor, 1,8-cineole, geraniol, camphene, methyl cinnamate, L-limonene and linalool as the major components, while methyl chavicol, α-bergamotene, 1,8-cineole and trans-β-ocimene were the main compounds of O. basilicum essential oil. Methyl cinnamate and camphor were the active components of B. pandurata oil, whereas methyl chavicol was the active component of O. basilicum oil by exhibiting the oocysticidal effect against E. tenella with IC50 values of 0.008, 0.023 and 0.054 mg/ml, respectively. Furthermore, B. pandurata and O. basilicum oils also showed a strong cytotoxic property against coccidia with more than 70% inhibition of sporozoite invasion in MDBK cell lines, and their IC50 were 0.004 and 0.004 mg/ml, respectively. Methyl cinnamate as well as camphor from B. pandurata and methyl chavicol from O. basilicum were also effective with IC50 values of 0.029, 0.023, and 0.022 mg/ml, respectively.