Publication: Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus
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Issued Date
2016-08-01
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ISSN
18790984
01660934
01660934
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2-s2.0-84965052635
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Virological Methods. Vol.234, (2016), 174-177
Suggested Citation
Rattiyaporn Kanlaya, Visith Thongboonkerd Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus. Journal of Virological Methods. Vol.234, (2016), 174-177. doi:10.1016/j.jviromet.2016.04.023 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/40782
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Title
Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus
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Abstract
© 2016 Elsevier B.V. Conventional method to purify/concentrate dengue virus (DENV) is time-consuming with low virus recovery yield. Herein, we applied cellufine sulfate column chromatography to purify/concentrate DENV based on the mimicry between heparan sulfate and DENV envelope protein. Comparative analysis demonstrated that this new method offered higher purity (as determined by less contamination of bovine serum albumin) and recovery yield (as determined by greater infectivity). Moreover, overall duration used for cellufine sulfate column chromatography to purify/concentrate DENV was approximately 1/20 of that of conventional method. Therefore, cellufine sulfate column chromatography serves as a simple, rapid, and effective alternative method for DENV purification/concentration.