Publication: Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures
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Issued Date
2016-01-01
Resource Type
ISSN
18783503
00359203
00359203
Other identifier(s)
2-s2.0-85014381547
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Mahidol University
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SCOPUS
Bibliographic Citation
Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.110, No.11 (2016), 670-672
Suggested Citation
Adul Dulsuk, Suporn Paksanont, Adisak Sangchankoom, Peeraya Ekchariyawat, Rungnapa Phunpang, Yaowaruk Jutrakul, Narisara Chantratita, T. Eoin West Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures. Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.110, No.11 (2016), 670-672. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/40858
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Title
Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures
Abstract
© The Author 2016. Background: Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis. Methods: We tested the diagnostic accuracy of the IFA on 545 blood cultures positive for Gram-negative organisms at Udon Thani Hospital, Thailand, between June 2015 and August 2016. Results: Sensitivity of the IFA was 100% and specificity was 99.6%. The median decrease in time to pathogen identification between the IFA result and routine methods was 28 h (IQR 25-51), p < 0.0001. Conclusions: The IFA accurately expedites the diagnosis of melioidosis.