Publication: Comparative proteomic analysis of Chlamydomonas reinhardtii control and a salinity-tolerant strain revealed a differential protein expression pattern
Issued Date
2017-11-01
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ISSN
14322048
00320935
00320935
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2-s2.0-85022043267
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Mahidol University
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SCOPUS
Bibliographic Citation
Planta. Vol.246, No.5 (2017), 843-856
Suggested Citation
Sayamon Sithtisarn, Kittisak Yokthongwattana, Bancha Mahong, Sittiruk Roytrakul, Atchara Paemanee, Narumon Phaonakrop, Chotika Yokthongwattana Comparative proteomic analysis of Chlamydomonas reinhardtii control and a salinity-tolerant strain revealed a differential protein expression pattern. Planta. Vol.246, No.5 (2017), 843-856. doi:10.1007/s00425-017-2734-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/41318
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Title
Comparative proteomic analysis of Chlamydomonas reinhardtii control and a salinity-tolerant strain revealed a differential protein expression pattern
Abstract
© 2017, Springer-Verlag GmbH Germany. Main conclusion: Proteins involved in membrane transport and trafficking, stress and defense, iron uptake and metabolism, as well as proteolytic enzymes, were remarkably up-regulated in the salinity-tolerant strain ofChlamydomonas reinhardtii. Excessive concentration of NaCl in the environment can cause adverse effects on plants and microalgae. Successful adaptation of plants to long-term salinity stress requires complex cellular adjustments at different levels from molecular, biochemical and physiological processes. In this study, we developed a salinity-tolerant strain (ST) of the model unicellular green alga, Chlamydomonas reinhardtii, capable of growing in medium containing 300 mM NaCl. Comparative proteomic analyses were performed to assess differential protein expression pattern between the ST and the control progenitor cells. Proteins involved in membrane transport and trafficking, stress and defense, iron uptake and metabolism, as well as protein degradation, were remarkably up-regulated in the ST cells, suggesting the importance of these processes in acclimation mechanisms to salinity stress. Moreover, 2-DE-based proteomic also revealed putative salinity-specific post-translational modifications (PTMs) on several important housekeeping proteins. Discussions were made regarding the roles of these differentially expressed proteins and the putative PTMs in cellular adaptation to long-term salinity stress.