Publication: High-performance liquid chromatography with time-programmed fluorescence detection for the quantification of Levofloxacin in human plasma and cerebrospinal fluid in adults with tuberculous meningitis
Issued Date
2017-09-01
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ISSN
1873376X
15700232
15700232
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2-s2.0-85025606536
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. Vol.1061-1062, (2017), 256-262
Suggested Citation
Pham Van Toi, Thomas Pouplin, Nguyen Duc Khanh Tho, Pham Nguyen Phuong, Tran Thi Hong Chau, Nguyen Thuy Thuong Thuong, Dorothee Heemskerk, Tran Tinh Hien, Guy E. Thwaites High-performance liquid chromatography with time-programmed fluorescence detection for the quantification of Levofloxacin in human plasma and cerebrospinal fluid in adults with tuberculous meningitis. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. Vol.1061-1062, (2017), 256-262. doi:10.1016/j.jchromb.2017.07.032 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/41736
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Title
High-performance liquid chromatography with time-programmed fluorescence detection for the quantification of Levofloxacin in human plasma and cerebrospinal fluid in adults with tuberculous meningitis
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Abstract
© 2017 The Authors An accurate and reliable high-performance liquid chromatography with time-programmed fluorescence detection was developed and validated to measure levofloxacin in human plasma and cerebrospinal fluid (CSF). After solid phase extraction process using Evolute®ABN 96 fixed well plate; levofloxacin and internal standard-enoxacin were separated using a mobile phase consisting of phosphate buffer 10 mM with 0.025% triethylamine pH 3.0 - acetonitrile (88:12, v/v) on a Purosphere RP-8e column (5 μm, 125 × 4.0mm) at a flow rate of 1.2 mL/min at 35 °C. The excitation/emission wavelengths were set to 269/400 nm and 294/500 nm, for enoxacin and levofloxacin, respectively. The method was linear over the concentration range of 0.02 to 20.0 μg/mL with a limit of detection of 0.01 μg/mL. The relative standard deviation of intra-assay and inter-assay precision for levofloxacin at four quality controls concentrations (0.02, 0.06, 3.0 and 15.0 μg/mL) were less than 7% and the accuracies ranged from 96.75% to 101.9% in plasma, and from 93.00% to 98.67% in CSF. The validated method was successfully applied to quantify levofloxacin in a considerable quantity of plasma (826) and CSF (477) samples collected from 232 tuberculous meningitis patients, and the preliminary intensive pharmacokinetics analysis from 14 tuberculous meningitis patients in Vietnam is described in this paper.