Publication:
Generation of induced pluripotent stem cells from peripheral blood CD34 + hematopoietic progenitors of a 31 year old healthy woman

Issued Date

2017-04-01

Resource Type

Article

ISSN

18767753
18735061

DOI

10.1016/j.scr.2017.02.013

Other identifier(s)

2-s2.0-85015644019

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Stem Cell Research. Vol.20, (2017), 91-93

Suggested Citation

Amornrat Tangprasittipap, Bunyada Jittorntrum, Wasinee Wongkummool, Narisorn Kitiyanant, Alisa Tubsuwan Generation of induced pluripotent stem cells from peripheral blood CD34 + hematopoietic progenitors of a 31 year old healthy woman. Stem Cell Research. Vol.20, (2017), 91-93. doi:10.1016/j.scr.2017.02.013 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/41936

Research Projects

Organizational Units

Authors

Journal Issue

Thesis

Title

Generation of induced pluripotent stem cells from peripheral blood CD34 + hematopoietic progenitors of a 31 year old healthy woman

Author(s)

Amornrat Tangprasittipap
 Bunyada Jittorntrum
 Wasinee Wongkummool
 Narisorn Kitiyanant
 Alisa Tubsuwan

Other Contributor(s)

Mahidol University

Abstract

© 2017 The Authors The MUi019-A human induced pluripotent stem cell line was generated from peripheral blood CD34 + hematopoietic progenitors of a healthy woman using a non-integrative reprogramming method. Episomal vectors carrying reprogramming factors OCT4, SOX2, KLF4, L-MYC, LIN28, and shRNA of TP53 and EBNA-1 were delivered using electroporation. The iPSC line can be used as a control in studying disease mechanisms. Furthermore, gene editing approaches can be used to introduce specific mutations into the MUi019-A to model disease while the cell type affected by the disease is inaccessible.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/41936

Collections

Scopus 2016-2017