Publication: Mutation analysis of isocitrate dehydrogenase (IDH1/2) and DNA methyltransferase 3A (DNMT3A) in Thai patients with newly diagnosed acute myeloid leukemia
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Issued Date
2017-02-01
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ISSN
2476762X
15137368
15137368
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2-s2.0-85016446900
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Cancer Prevention. Vol.18, No.2 (2017), 413-420
Suggested Citation
Tanasan Sirirat, Suporn Chuncharunee, Pimjai Nipaluk, Teerapong Siriboonpiputtana, Takol Chareonsirisuthigul, Nittaya Limsuwannachot, Budsaba Rerkamnuaychoke Mutation analysis of isocitrate dehydrogenase (IDH1/2) and DNA methyltransferase 3A (DNMT3A) in Thai patients with newly diagnosed acute myeloid leukemia. Asian Pacific Journal of Cancer Prevention. Vol.18, No.2 (2017), 413-420. doi:10.22034/APJCP.2017.18.2.413 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/41961
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Title
Mutation analysis of isocitrate dehydrogenase (IDH1/2) and DNA methyltransferase 3A (DNMT3A) in Thai patients with newly diagnosed acute myeloid leukemia
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Abstract
Acute myeloid leukemia (AML) is a clonal hematopoietic stem/progenitor cell disorder which features several genetic mutations. Recurrent genetic alterations identified in AML are recognized as causes of the disease, finding application as diagnostic, prognostic and monitoring markers, with potential use as targets for cancer therapy. Here, we performed a pyrosequencing technique to investigate common mutations of IDH1, IDH2 and DNMT3A in 81 newly diagnosed AML patients. The prevalences of IDH1, IDH2 and DNMT3A mutations were 6.2%, 18.5%, and 7.4%, respectively. In addition, exclusive mutations in IDH1 codon 132 (R132H, R132C, R132G and R132S) were identified in all IDH1-mutated cases indicating that these are strongly associated with AML. Interestingly, higher median blast cell counts were significantly associated with IDH1/2 and DNMT3A mutations. In summary, we could establish a routine robust pyrosequencing method to detect common mutations in IDH1/2 and DNMT3A and demonstrate the frequency of those mutations in adult Thai AML patients.