Publication: A Phenylurea Cytokinin, CPPU, Elevated Reducing Sugar and Correlated to Andrographolide Contents in Leaves of Andrographis paniculata (Burm. F.) Wall. Ex Nees
2
Issued Date
2017-02-01
Resource Type
ISSN
15590291
02732289
02732289
Other identifier(s)
2-s2.0-84986309698
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Applied Biochemistry and Biotechnology. Vol.181, No.2 (2017), 638-649
Suggested Citation
Phapawee Worakan, Netiya Karaket, Nuchada Maneejantra, Kanyaratt Supaibulwatana A Phenylurea Cytokinin, CPPU, Elevated Reducing Sugar and Correlated to Andrographolide Contents in Leaves of Andrographis paniculata (Burm. F.) Wall. Ex Nees. Applied Biochemistry and Biotechnology. Vol.181, No.2 (2017), 638-649. doi:10.1007/s12010-016-2238-x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/42036
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
A Phenylurea Cytokinin, CPPU, Elevated Reducing Sugar and Correlated to Andrographolide Contents in Leaves of Andrographis paniculata (Burm. F.) Wall. Ex Nees
Other Contributor(s)
Abstract
© 2016, Springer Science+Business Media New York. Cytokinins are phytohormones that play multiple roles to control plant growth and development. In this study, leaf biomass and the production of andrographolide compounds in a medicinal plant Andrographis paniculata were significantly increased after exogenously treating with the synthetic cytokinin cytokinin-1-(2-chloro-4-pyridyl)-3-phenylurea (CPPU) at 0 (water), 5, or 10 mg L−1and observed the results for 24 h, 48 h, and 7 days of treatment. It was found that CPPU could significantly enhance new axillary bud formation and further promote branching 4.6–5.6-fold higher, resulting in higher fresh weight (FW) and dry weight (DW) than the control. Application of CPPU at 5 mg L−1significantly promoted the highest contents of total reducing sugar at 2.5-fold in leaves and at 1.5-fold in roots. Although treatments of CPPU significantly affected the increasing contents of chlorophyll and carotenoid (1.2–1.6-fold), CPPU at 10 mg L−1slightly caused leaf stress and chlorophyll reduction. Interestingly, 5 mg L−1CPPU could enhance andrographolide content, an active anti-infectious compound in Andrographis paniculata (2.2-fold higher than the control) that reached the highest content at 24 h after treatment. This study suggested that CPPU should be suitable for field application to promote leaf yields and induce the production of useful pharmaceutical compounds in Andrographis paniculata.