Publication: Fermentation of hydrogen, 1,3-propanediol and ethanol from glycerol as affected by organic loading rate using up-flow anaerobic sludge blanket (UASB) reactor
Issued Date
2017-01-01
Resource Type
ISSN
03603199
Other identifier(s)
2-s2.0-85020449243
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Hydrogen Energy. Vol.42, No.45 (2017), 27558-27569
Suggested Citation
Sureewan Sittijunda, Alissara Reungsang Fermentation of hydrogen, 1,3-propanediol and ethanol from glycerol as affected by organic loading rate using up-flow anaerobic sludge blanket (UASB) reactor. International Journal of Hydrogen Energy. Vol.42, No.45 (2017), 27558-27569. doi:10.1016/j.ijhydene.2017.05.149 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/42529
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Fermentation of hydrogen, 1,3-propanediol and ethanol from glycerol as affected by organic loading rate using up-flow anaerobic sludge blanket (UASB) reactor
Author(s)
Other Contributor(s)
Abstract
© 2017 Hydrogen Energy Publications LLC The influences of organic loading rate (OLR) on the production of biohydrogen, 1,3-propanediol (1,3-PD) and ethanol from glycerol in an up-flow anaerobic sludge blanket (UASB) by anaerobic mixed cultures were investigated. UASB reactor was firstly operated using pure glycerol as the substrate at various OLRs of 25, 37.5, 50, 62.5 g/L d. Results showed that a change in OLR led to a change in hydrogen production, hydrogen production rate (HPR), hydrogen yield (HY), 1,3-PD and ethanol production as well as glycerol consumption, and microbial community. The optimal OLR for hydrogen and ethanol production was found to be 50 g/L d when the UASB was fed with pure glycerol, while the optimal OLR for 1,3-PD production was 62.5 g/L d. After the optimum OLR was obtained, the reactor was fed with crude glycerol. Maximum hydrogen, ethanol and 1,3-PD production from crude glycerol was obtained at the same OLR as of pure glycerol. A maximum hydrogen production, HY, ethanol and 1,3-PD production of 134.2 mmol/L, 579.8 mmol H2/mol glycerol, 78.9 mmol/L and 60.9 mmol/L, respectively, were obtained when pure glycerol was used as the substrate. However, when crude glycerol was used as the substrate, a maximum hydrogen production, HY, ethanol and 1,3-PD production were lower than that of using pure glycerol as the substrate. Analysis of microbial community by polymerase chain reaction-denaturing gradient gel electrophoresis indicated that the predominant hydrogen producers at the optimum OLR of 50 g/L d, when crude glycerol was used as the substrate, were Clostridium sp., Enterobacter sp., uncultured Firmicutes bacterium, and uncultured Actinobacterium while Enterobacter sp., Klebsiella sp., were the predominant 1,3-PD producers and Clostridium sp., as well as Enterobacter sp., were the predominant ethanol producers.