Publication: Magnetic Nanoparticles PCR Enzyme-Linked Gene Assay for Quantitative Detection of BCR/ABL Fusion Gene in Chronic Myelogenous Leukemia
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Issued Date
2016-09-01
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10982825
08878013
08878013
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2-s2.0-84989244083
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Clinical Laboratory Analysis. Vol.30, No.5 (2016), 534-542
Suggested Citation
Yanaphat Manthawornsiri, Duangporn Polpanich, Vichanan Yamkamon, Raweewan Thiramanas, Suradej Hongeng, Budsaba Rerkamnuaychoke, Saengsuree Jootar, Pramuan Tangboriboonrat, Kulachart Jangpatarapongsa Magnetic Nanoparticles PCR Enzyme-Linked Gene Assay for Quantitative Detection of BCR/ABL Fusion Gene in Chronic Myelogenous Leukemia. Journal of Clinical Laboratory Analysis. Vol.30, No.5 (2016), 534-542. doi:10.1002/jcla.21899 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/42906
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Title
Magnetic Nanoparticles PCR Enzyme-Linked Gene Assay for Quantitative Detection of BCR/ABL Fusion Gene in Chronic Myelogenous Leukemia
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Abstract
© 2015 Wiley Periodicals, Inc. Background: Magnetic nanoparticles (MNPs) have been widely used in medical diagnostic research. In this work, two technologies, MNPs and polymerase chain reaction (PCR), were combined to increase detection sensitivity and specificity. A novel technique based on the MNPs-PCR enzyme-linked gene assay (MELGA) was developed for detection of the BCR/ABL abnormal gene in chronic myelogenous leukemia (CML) patients. Methods: An MNPs-labeled BCR forward primer and a biotin-labeled ABL reverse primer were used to specifically amplify the target gene. After magnetic separation, the PCR product bound to MNPs labeled with streptavidin-conjugated horseradish peroxidase was incubated with the peroxidase substrate and hydrogen peroxide to generate the colorimetric signal. Results: When compared with real-time quantitative-PCR (RQ-PCR), the MELGA technique exhibited an increased sensitivity of <1 fg with high specificity for the BCR/ABL fusion gene in CML patients. In addition, MELGA colorimetric results correlated well with the number of copies obtained from RQ-PCR. Conclusion: This simple and cost-effective technique is suitable for monitoring CML patients during targeted therapy (tyrosine kinase inhibitors) especially in rural hospitals.